Materials used for modified seed germination inhibition test. (A) Used mineral water container (B) Toilet tissue paper (C) 10 ml syringe without needle for measuring urine (d) Spices tray 

Seed germination inhibition test in the conventional method. Picture shows the germination level at 72 hours setting of the experiment. A. Urine collected from pregnant buffalo confirmed through rectal palpation, B. Tap water control, C. Distilled water control, D. Urine collected from non- pregnant buffalo. 

Seed germination inhibition test with some modifications. Pictures show the germination level at different time frame of the experiment. Four separate pictures represent different time points such as, (A) 3hr after setting (B) 24 hr after setting (C) 48 hr after setting (D) 72 hr after setting. One picture expresses four distinct experiment using different samples, (W) Tap water control (S1), Urine collected from pregnant buffaloes confirmed through rectal palpation (NP), Urine collected from non-pregnant buffaloes (S2), Urine collected from pregnant buffalo confirmed through rectal palpation of same animal S1. 

Germination inhibition percentages of sorghum seeds in different groups at 48 hours and 72 hours of the setting of modified seed germination inhibition experiment. The data represent the averages from quadruplicate samples based on thirty (30) identical experiments, and the error bars denote the standard deviations. Significant differences were accepted at P <0.05. Here * indicates significant differences between urine of pregnant animal and urine of non-pregnant animal, and ** indicates significant differences between urine of pregnant animal and water control. 

Detection of pregnancy using the chemical method. (A) Left two tubes indicate positive and negative results of milk copper sulphate test (b) Right two tubes indicate positive and negative results of urine barium chloride test.