Clinical manifestations and PM lesions of laying chickens suspected to be infected with AIVs and NDV. (A) severe congestion in comb, wattle and face edema in 28week flock No.13. Belbais; (B) severe hemorrhage and congestion of ovary; (C) severe congestion in spleen and ascites in 28 week flock No.19. Hehia; (D) hemorrhage and congestion in duodenum, pancreas in 25 week flock No.5 Talraq. 

Phylogenetic tree of amino acid sequence of the hemagglutinin genes of three field isolate of avian influenza subtype H9N2 (black circle) viruses isolated in Egypt during 2017–2019 and with reference strains from GenBank. 

Phylogenic tree of amino acid sequence of NA gene of three field isolate subtype H9N2 (black circle) with other reference strains in gene bank. 

Phylogenic tree of amino acid sequence of HA gene of two field isolates highly pathogenicH5N8 (black star) with other reference strains in gene bank. 

Phylogenic tree of amino acid sequence of NA gene of two field isolate subtype H5N8 (black circle) with other reference strains in gene bank. 

Phylogenetic tree of selected field NDV (3 isolates) indicated by red circle with other related reference strains of NDV in gene bank. 

Amino acid identity of HA and NA genes of H5N8 and H9N2.