Uniplex PCR to amplify some antimicrobial resistance genes in 4 S. aureus strains from ready to eat food. A) PCR products of mec A gene. P: positive control isolate; N: negative control isolate of Escherichia coli; L: Ladder 100 bp; Lanes 1, 2, 3 and 4: positive strains for mec A gene (310 bp) isolated from kofta, shawrma, sausage and fried shrimp, respectively; B) PCR products of bla Z gene. The four S. aureus isolates (lanes1-4) were positive PCR product of 173 bp; C) PCR products of aac(6’)aph (2’’) gene. The lanes1-4 were with amplicon of 491 bp; D) PCR products of mph C gene. The two S. aureus isolates from kofta and shawarma (Lanes 1 and 2) were negative, while other two S. aureus strains from sausage and fried shrimp (lanes 3and4) were positive with PCR product of 722 bp.
 

Agarose gel electrophoresies of two virulence genes (clf A and spa) in 4 strains of S. aureus isolated from ready to eat food. A) PCR products of clf A gene. P: positive control isolate; N: negative control; L: Ladder 100 bp; Lanes 1- 4: positive strains for clfA gene (638 bp) isolated from kofta, shawarma, sausage and fried shrimp, respectively; B) PCR products of spa gene. The four S. aureus isolates (lanes1-4) were positive with amplicons of 226 bp.