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South Asian Journal of Life Sciences

Research Article
S. Asian J. Life Sci. 6(1): 1-6
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Anwar Ali Turi1, Abdur Rahman2, Ijaz Ali1, Rafiullah1, Abdul Sajid3, Khalid Khan1,Imtiaz Ali Shah1, Muhammad Asif Gondal4, Ameen-ur-Rashid4*, Siraj Ahmed5, Inamullah Wazir6, Sohail Khan1, Arifullah Khan

1Veterinary Research Institute Peshawar; 2Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar; 3The Abdul Wali Khan University Mardan; 4Department of Biosciences, COMSATS Institute of Information Technology, Islamabad; 5Department of Microbiology, Abasyn University Peshawar; 6Institute of Microbiology, The University of Agriculture, Faisalabad, Pakistan.

Abstract | Anaplasmosis is disease of livestock having pandemic in nature. In the present study the specificity and sensitivity of i-ELISA and RT PCR was investigated in the samples collected from District Peshawar and Lakki Marwat. A total of 900 samples were collected from three species, Cattle, Buffalo and Sheep from two transects Peshawar and Lakki Marwat for determination of rate of Anaplasma marginale through i-ELISA and RT PCR. Overall rate for Anaplasmosis was 37.7% (339/900) for all three techniques, whereas techniques wise the rate of Anaplasma marginale was 28.7% and 34.8 % through i-ELISA and Real-time PCR, respectively. Majority of the Cattle (41.6 %) were found positive for A. marginale as compared to 23.7% and 16.2% in Sheep and Buffalo respectively. Transects were significantly different at P <0.05 in term of rate of A. marginale. Transect wise distribution of anaplasmosis was higher in Lakki Marwat (41.5%) as compared to Peshawar (30%). Gender wise rate was higher in female through i-ELISA (57.96%) and RT PCR (64.33%) as compared to male individuals having 16.27% and 23.25% rate, respectively. A significant difference was found in seasonality of tick infestation, with the high infestations in summer as compare to winter.

Keywords | Anaplasma marginale, Cattle, Buffalo, Sheep, Peshawar, Lakki Marwat, i-ELISA, RT PCR