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Journal of Infection and Molecular Biology

Research Article
J. Inf. Mol. Biol. 5(1): 1-6
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Shakeel Hussain, Kamran Ashraf, Naveed Anwar, Muhammd Ameen Jamal, Huma Naeem, Nisar Ahmad, Aziz-Ul-Rahman 

1Department of Wildlife Conservation and Utilization, Northest Forestry University Harbin 150069, China; 2Department of Parasitology, University of Veterinary and Animal Sciences Lahore 54600, Pakistan; 3State Key Laboratory of Veterinary Biotechnology, Harbin VeterinaryResearch Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China; 4Department of Animal Breeding Genetics and Reproduction, Yunnan Agricultural University, Kunming 650201, Yunnan, PR China; 5Department of Microbiology, University of Veterinary and Animal Sciences Lahore 54600, Pakistan.
Abstract | A comparative study was conducted to evaluate the incidence of Babeisa bovis (B. bovis) infection in crossbred and indigenous cattle with relation to age and breedin Lahore, Pakistan using microscopic examination and PCR technique. A total of 60 blood samples of clinically ill cattle were obtained from different dairy farms. An overall prevalence of 8.3% by PCR and 6.67% by microscopy were recorded for B. bovis. The crossbred cattle showed significantly (p<0.05) high prevalence of 14.33% and 10% by using PCR and microscopy, respectively, compared to indigenous cattle with 3.33% using both diagnostic techniques. Among the different age group, animals with >2 year of age had a higher prevalence of 15.38% than 5.26% in 1-2 year of animals and at least 0.00% in <1 year of animals. It was concluded from this study that PCR found as an efficient technique rather than microscopy for diagnosis of babesiosis in carrier state infectionin those regions where haemoparasites are endemic in the cattle population.The findings of this work may aid in the prevention or control of the babesiosis.

Keywords | Prevalence, Babesia bovis, Indigenous cattle, Crossbred cattle, Microscopy, PCR.