Abstract | A cross sectional study was conducted to evaluate current immune status of various commercial and domesticated birds, including broiler, layers, backyard poultry and other domesticated birds (Pigeon, Peacock, Turkey). Of the total serum samples (n = 452) obtained from 113 flocks, only 372 samples were seropositive to NDV using competitive ELISA. An overall prevalence of 82.3% was noted in studied birds across the country. Seropositivity was more frequent in Khyber Pakhtunkhwa (84.5%) followed by Baluchistan (83.3%), Punjab (82.3%) and Sindh (79.4%). Among the bird types, seropositivity was higher in domesticated wild birds followed by rural birds, commercial broiler and layers (88.6%, 83.9%, 81.9% and 73.3%), respectively. An incresaed seroprevalence provides a clear evidence of exposure to NDV with history of previous outbreak in these farms. Except from commercial broiler and layers, ND vaccine was not given to the birds and high titer coupled with disease occurrence notifies the vaccine failure in commercial birds. In the absence of the vaccine, the results provide a clear evidence of exposure to NDV to wild domesticated birds. Further studies are required to determine the strains circulating for appropriate preventive and control measures.
Keywords | Newcastle disease virus, Khyber Pakhtunkhwa, Punjab, Baluchistan, Sindh, Seropositivity, cELISA