Abstract | The Current study was conducted to investigate anti-microbial resistance pattern of Escherichia coli (E. coli) isolated from hospital effluent and the occurrence of tetracycline resistance determinant genes: tet (A), tet (B) and tet (C) in tetracycline-resistant isolates. Effluent samples were collected from 15 randomly selected hospitals of Chittagong Metropolitan area for the isolation of E. coli based on cultural and biochemical properties. The isolated E. coli were screened for the anti-microbial susceptibility against 10 commonly used anti-microbials in the hospitals. Tetracycline-resistant isolates (13 out of 15) were employed for polymerase chain reaction (PCR) to determine the presence of tetracycline resistance determinant genes. E. coli isolates were resistant (100%) against amoxicillin and cephalothin followed by tetracycline (86.67%), sulphamethoxazole-trimethoprim (80%), ceftriaxone (73.33%), nalidixic acid (66.67%), enrofloxacin (66.67%) and chloramphenicol (20%). Isolates were sensitive against gentamycin (86.67%) followed by neomycin (73.33%), chloramphenicol (66.67%), ceftriaxone (20%) and sulphamethoxazole-trimethoprim (20%). Moreover, all isolates showed multi-drug resistance pattern. The prevalence of tetracycline resistance determinants were 53.85%, 15.38% and 0% for tet (A), tet (B) and tet (C) genes respectively. Both tet (A) and tet (B) genes were positive in 15.38% isolates. None of the isolates possessed all three genes or tet (B) and tet (C) genes or tet (A) and tet (C) genes collectively. A 53.85% isolates possessed one or more of the tested genes whereas 46.15% isolates had no tested genes. The study revealed that hospital effluent might be one of the major sources of the multi-drug resistant E. coli in environment and, to overcome this problem, the hospital effluent should be treated efficiently.
Keywords | Anti-microbial resistance, E. coli, Hospital effluent, tet, PCR