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Journal of Infection and Molecular Biology

Research Article
J. Inf. Mol. Biol. 1 (3): 41 - 45
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Tasra Bibi1*, Asim Aslam1, Shafqat Fatima Rehmani2, Arfat Yousaf Shaheen3, Nadia Mukhtar3, Abdul Wajid4, Saira Jabeen3, Suliman Qadir Afridi3, Hassan Anjum Shahid5
1Department of Pathology, University of Veterinary and Animal Sciences Lahore 54000, 2Quality Operation Laboratory (QOL), University of Veterinary and Animal Sciences Lahore 54000, 3Department of Microbiology, University of Veterinary and Animal Sciences Lahore 54000, 4Institute of Biochemistry and Biotechnology (IBBt), University of Veterinary and Animal Sciences Lahore 54000, 5King Edward Medical University, Lahore, Pakistan
*Corresponding author: tasraanjum@gmail.com

 

ABSTRACT
One hundred ninety five (195) chicks were used to collect three different lymphoid organs i.e Thymus, Bursa of Fabricious and Harderian gland after vaccinating with Newcastle disease virus to observe the histopathological changes. The chicks were divided into three groups and vaccinated via coarse spry, eye drop and drinking water at the age of five days and then boasted at twenty six days of age. The histopathological changes show high number of Plasma cells in these lymphoid organs. The segregation of plasma cells was significantly higher in Harderian gland using NDV vaccine by eye drop method. All of the three routes of immunization provided comparable protection against the challenged infection at 6 weeks of age. However, the coarse spray method of vaccination provided significantly high level of circulating antibodies as compared to ocular and drinking water vaccination.

Key Words: Newcastle disease, spray, ocular route, drinking water, lymphoid organs