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Journal of Infection and Molecular Biology

Research Article
J. Inf. Mol. Biol. 3 (3): 75 - 80
http://dx.doi.org/10.14737/journal.jimb/2015/3.3.75.80
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Muhammad Sohail1, 2*, Qamar Sultana2, 6, Karam Rasool2, 4, Samreen Sarwar3, Abdul Basit2,5, Muhammad Khalid2

1Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54000, Pakistan; 2Chughtais Lahore Lab, Lahore, 54660, Pakistan; 3University of Health Sciences Lahore, 54000, Pakistan; 4Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 5Forensic Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan; 6Department of Pathology, Sir Ganga Ram Hospital, Queen’s Road, Lahore, Pakistan.

*Correspondence | Muhammad Sohail, 10-Jail Road, Chughtais Lahore Lab, Lahore, Pakistan; Email: drsohailmmg@gmail.com


Abstract
Bacteremia is associated with high rates of morbidity and mortality. For early diagnosis of bacteremia; prior use of antibiotics, suboptimal volume of blood sample and improper microbiological techniques are strongly condemned. In time-diagnosis of bacteremia can reduce the antibiotic resistance, hospital stay and cost to the treatment. Blood samples from patients with history of fever in conjunction with clinical signs and symptoms of bacteremia (from July 2013 to July 2014) were processed using BACTEC 9050 automated system. ESR (erythrocyte sedimentation rate), CRP (C- reactive protein) and TLC (total leukocyte count) were also measured, respectively. Out of 3559 samples, 525 (14.75%) samples were positive for bacterial growth. Among positive blood cultures, the pathogens isolated were coagulase negative Staphylococcus aureus (36.38 %), E. coli (18.28%), Methicillin sensitive Staphylococcus aureus (MSSA) (7.04%), Strept. fecalis (5.9%), Salmonella typhi (6.09%), Candida species (4.95%), Pseudomonas species (4.38%), Klebsiella pneumonia (4%), Acinetobacter species (3.42%), Salmonella paratyphi A (2.85%), Methicillin resistant Staphylococcus aureus (MRSA) (2.67%), Citrobacter species (2.47%), Streptococcus species other than Strept. faecalis (1.52%), Xanthomonas maltophilia (0.57%), Vibrio species (0.19%), Yersinia species (0.19%) and Klebsiella oxytoca (0.19%). ESR, CRP and TLC showed poor correlation with bacteremic patients. In addition, increased antimicrobial resistance was observed in all pathogens isolated. ESR, CRP and TLC were considered as preliminary indicator of infections having low specificity and sensitivity in blood stream infections.

Keywords | Antimicrobial susceptibility testing, Bacteremia, Total leukocyte count, Erythrocyte sedimentation rate, C-reactive protein

Editor | Tahir Yaqub, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Received | July 02, 2015; Revised | October 11, 2015; Accepted | October 14, 2015; Published | November 14, 2015

Citation | Sohail M, Sultana Q, Rasool K, Sarwar S, Basit A, Khalid M (2015). Bacteremia prediction by inflammatory factors and recent trend in drug resistance of bacteria isolated from blood stream infection. J. Inf. Mol. Biol. 3(3): 75-80.

ISSN (Online) | 2307-5465; ISSN (Print) | 2307-5716

Copyright © 2015 Sohail et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.