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Journal of Infection and Molecular Biology

Research Article
J. Inf. Mol. Biol. 3 (2): 34 - 46
http://dx.doi.org/10.14737/journal.jimb/2015/3.2.34.46
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Noha Mohamed Osman1,2*, Ahlam Ahmed Abou Mossallam2, Fawzy Reyad El Seedy3, Eman Roshdy Mahfouz2, Rui Li4, Michal Mikula5

1Department of Molecular and Computational Biology, University of Southern California, 1050 Childs Way RRI 316 Los Angeles, CA 90089, USA; 2Department of Cell Biology, Genetic Engineering Division, National Research Center, Dokki, Giza, Egypt; 3Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt; 4Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA; 5Department of Oncological Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.

*Correspondence | Noha Mohamed Osman, University of Southern California, Los Angeles, USA; Email: nmo_138@usc.edu, Osman.noha@yahoo.com

Abstract
Toll-like receptors (TLRs) are a vital component of innate immune system and perform an important role in disease resistance through recognition of pathogen associated molecular patterns (PAMPs) and initiation of a convenient immune response. Endometritis is the most important disease causing infertility in cattle and buffalo and TLRs play a critical role in elimination of that disease. The present study aims to characterize the transcripts of TLRs: TLR1, TLR2, TLR4, TLR6 and TLR10 in Bubalus bubalis with endometritis. Quantitative real-time (q-PCR) assays were performed to detect transcript expression profiles of these TLRs in liver, mammary gland, ovary and uterus of Bubalus bubalis with and without endometritis. The result showed that the transcript profiles of TLRs especially TLR2 and TLR4 were significantly differences in mammary glands, ovary and uterus but on the othe hands, there is no significant difference in the transcript profile of TLRs in liver with or without endometritis. TLRs nucleotide sequences have more than 97% nucleotide homology between Bubalus bubalis with and without endometritis. Simple modular architecture tool (SMART) analysis revealed the presence of TIR domains and different numbers of leucine rich repeat motifs in tested TLRs. In conclusion, for animals infected with endometritis, there was a significant difference of TLR2 and TLR4 in uterine tissue compared to uninfected animals. Therefore, it is suggested to consider the TLRs results as detection markers for endometritis.

Keywords | Toll-Like receptors (TLRs), Bubalus bubalis, Endometritis, Gene expression