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Journal of Infection and Molecular Biology

Short Communication
J. Inf. Mol. Biol. 3 (2): 28 - 33
http://dx.doi.org/10.14737/journal.jimb/2015/3.2.28.33
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Hashaam Habib1, Shafqat Fatima Rehmani2, Nadia Mukhtar1, Tasra Bibi1, Abdul Wajid2,3

1Department of Microbiology; 2Quality Operations Laboratory and 3Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan.

*Correspondence | Shafqat Fatima Rehmani, University of Veterinary and Animal Sciences, Lahore, Pakistan; Email: rehmani.shafqat@uvas.edu.pk

Abstract
Newcastle disease (ND) is a fatal and contagious viral disease of many avian species predominantly domestic poultry that creates a constant threat to the poultry industry around the world including Pakistan. The efficient diagnosis of NDV strains become a challenge due to complex clinico-pathological picture and high genetic variability. In this study, one hundred ND suspected samples were collected from the proximity of Lahore district during 2010. Upon virus isolation, these samples were subjected to haemagglutination (HA) and haemagglutination inhibition (HI) tests for the confirmation of NDV. Ten confirmed samples were pathogenic as determined by ICPI and MDT. Five NDV isolates representing different geographical areas of Lahore were subjected for the amplification of full length hemagglutinin-neuraminidase (HN) gene (1892 bp). Subsequently, a partial HN (519 bp) was sequenced to characterized the NDV isolates. All the isolates had closed phylogenetic relationship with previously characterized Pakistani and Indonesian isolates within genotype VII. The present investigation provides essential information on the genetic nature of HN protein of NDV circulating in Pakistan and emphasizes the importance of study on disease diagnosis and control.

Keywords | Newcastle disease, ICPI, MDT, HN gene, Phylogenetic analysis