Journal of Infection and Molecular Biology

Research Article
J. Inf. Mol. Biol. 3 (1): 24 - 27
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Mukesh Bhakat1*, Tushar Kumar Mohanty2, Ashok Kumar Gupta1, Atish Kumar Chakravarty1, Pawan Singh1, Muzamil Abdullah1

1Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India;
2 Livestock Research Centre, Livestock Production Management, ICAR-National Dairy Research Institute, Karnal – 132001, Haryana, India.

*Correspondence | Mukesh Bhakat, ICAR-National Dairy Research Institute, Karnal, Haryana, India; Email:

Vaccination is one of the major anaphylactic stress factors that affect the semen quality. A study was conducted to evaluate the effect of Haemorrhagic Septicaemia (HS) and Black Quarter (BQ) vaccination stress on semen quality parameters of Murrah buffalo bulls in Artificial Breeding Research Centre, ICAR-NDRI, Karnal. Three Murrah buffalo bulls (MU), selected on the basis of those produced freezable quality semen and donated a minimum of four ejaculates each during pre-vaccination and post-vaccination period during experimental period. A total 24 ejaculates were taken before vaccination, which served as control and 24 ejaculates were collected after vaccination to study the effect of vaccination stress. Statistical analysis revealed that HS and BQ vaccination had significantly (P<0.05) adverse effect on mass activity (1.75±0.12 vs. 1.23±0.14), sperm concentration per ml (566.67±46.50 ×106/ml vs. 408.33±45.38 ×106/ml) and total sperm output per ejaculate (1624.79±159.14 vs. 964.58±153.12 ×106) in buffalo bulls. Ejaculate volume (2.82±0.28 vs. 2.54±0.28 ml) and total volume per day (4.23±0.55 vs. 4.07±0.57 ml) were not significantly (P>0.05) affected, whereas percent individual motility (IM) (49.54±0.15 vs. 38.01±0.11) was significantly (P>0.05) decreased. In this study, we found a decline in the mean values of all the seminal attributes under study after vaccination from pre-vaccination value. So, the spermiograms affected following vaccination suggests that in Murrah buffalo bulls, the semen collection and preservation should be suspended till normal fertility of sperm is restored to avoid the failure of conception from AI using such semen.

Keywords | HS and BQ vaccine, Mass activity, Ejaculate volume, Individual motility, Sperm concentration, Murrah buffalo, ICAR-NDRI