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Journal of Infection and Molecular Biology

Short Communication
J. Inf. Mol. Biol. 2 (4): 74 - 76
http://dx.doi.org/10.14737/jimb.2307-5465/2.4.74.76
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Muhammad Irfan2, Imran Ahmed2, Muhammad Shafee1*, Abdul Malik Tareen2, Muhammad Fazal Ur Rehman2, Shabbir Ahmad Khan2
1Center for Advanced Studies in Vaccinology & Biotechnology (CASVAB) University of Balochistan, Quetta, Pakistan; 2Department of Microbiology, University of Balochistan, Quetta, Pakistan
*Corresponding author: shafeedr73@gmail.com

ABSTRACT
Thermal injury is a serious problem that requires special management of patients in burn intensive care units. This study was designed to isolate the most common pathogens and study their antibiogram profile to commonly used antibiotics. A total of 100 wound samples were collected and subjected to microbial identification. All the samples were cultured on selective medias, cetrimide and mannitol salt agar for the isolation of Pseudomonas aeruginosa and Staphylococcus aureus. After confirmation through colony characteristics and biochemical tests, all the isolates were evaluated against commonly used antibiotics viz, Amikacin, Gentamycin, Amoxicillin and Ceftazidime, for their susceptibility using disc diffusion method. P. aeruginosa (37%) was the most common pathogen followed by Staphylococcus aureus (14%) respectively. Amikacin and Ofloxacin were found with highest zone of inhibition (18 mm and 24mm) against P. aeruginosa and S. aureus. This study explores the presence of these clinically important pathogens in burn wounds.

Key Words: Pseudomonas aeruginosa, Staphylococcus aureus, Prevalence, Burn wounds, Quetta