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Journal of Infection and Molecular Biology

Short Communication
J. Inf. Mol. Biol. 2 (3): 39 - 42
http://dx.doi.org/10.14737/jimb.2307-5465/2.3.39.42
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 Farzana Shaheen1, Masood Rabbani4, Zafar ul Ahsan Qureshi2, Mahmood ul Hassan3, Arfan Ahmad4, Fareeha Akhtar4, Suliman Qadir Afridi1, Hassaan bin Aslam5, Mehboob Akbar6, Tasra Bibi1, Nadia Mukhtar1*
1Quality Operations Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan; 2Veterinary Research Institute, Lahore Cantt, Pakistan; 3University of Agriculture Faisalabad, Pakistan; 4University Diagnostic Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan; 5Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 6Poultry Production Rajanpur, Livestock and Dairy Development Department, Punjab
*Corresponding author: nadiamukhtar84@yahoo.com

ABSTRACT
Rabies is an ancient zoonotic disease of mammals caused by a RNA virus within Rhabdoviridae family. Rabies virus surveillance in bats was performed in Pakistan during 2009 and 2010 through indirect fluorescence test (iFAT), Mice inoculation test (MIT) and reverse transcriptase–polymerase chain reaction (RT-PCR) techniques. One hundred oropharyngeal swabs and brain tissues of different bat species i.e; Taphozous nudiventris, Scotophilus heathii, Scotoecus pellidus, Pipistrellus pipistrellus and Scotophilus kohlil were collected from five areas named Lahore, Pattoki, Sheikhupura, Bahawalpur and Bahawalnagar of Punjab province, Pakistan. All the techniques were validated by suspected brain samples of dog, cow, mule, and known positive control. In the study, all insectivorous bats species were found negative to rabies virus.

Key Words: Lyssavirus, Surveillance, Bats, iFAT, MIT, RT–PCR