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Journal of Infection and Molecular Biology

Short Communication
J. Inf. Mol. Biol. 1 (4): 67 - 70
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Khawar Ali Shahzad1*, Fahim Ullah1, Khushi Muhammad2, Farzana Khatoon1, Mahmood Ul Hassan Qazi1, Ishfaq Ahmed1
1Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan; 2Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan
*Corresponding author: khawar7bar@yahoo.com

ABSTRACT
Urinary tract infection (UTI) is the most common problem in hospitalized and outdoor patients. It is mainly found in females because of the shortness of the urethra and closeness to anus, which facilitate entrance of fecal micro–flora to urinary tract. Aim of the study was to investigate the bacterial uro-pathogens and their antibiotic susceptibility in a tertiary care hospital, Peshawar, Pakistan. Urine samples (n = 200) were analyzed and cultured on cysteine lactose electrolyte deficient (CLED) medium. All the bacterial isolates were identified by conventional biochemical tests. Of the total, bacteria were isolated from 113 patients. In positive samples, 36 (31.9%) were male and 77 (68.1%) were female, whereas, 80 (70.8%) were hospitalized and 33 (29.2%) were walk in patients. E. coli was the dominant uro-pathogen 77 (68.1%) followed by Staphylococcus aureus 13 (11.5%), Proteus spp. 9(8.0%), Pseudomonas spp. 6 (5.3%), Klebsiella spp. 4(3.5%), and methicillin resistant Staphylococcus aureus 4 (3.5%). Antibiotic susceptibility test was performed by disc diffusion method according to clinical laboratories standard institute (CLSI). Bacterial isolates showed resistance to ampicillin (72.0%), ciprofloxacin (53.1%), norfloxacin (51.3%) and trimethoprim–sulfamethaxozole (53.1%). Bacterial spp. resistant to other antibiotics was also prevalent. Meropenem was the most effective antibiotic against all the bacterial isolates. In conclusion, high incidence of single and multiple antibiotic resistant bacterial strains is matter of enormous concern. Meropenem was the drug of choice to control urinary tract infections.

Key Words: Urinary tract infection, Multi drug resistant, E. coli