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Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 6(1): 41-46
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Devender Kumar, Govind Narayan Purohit

Department of Veterinary Gynecology and Obstetrics, College of Veterinary and Animal Sciences,, Rajasthan University of Veterinary and Animal Sciences, Bikaner Rajasthan India 334001.

Abstract | In order to evaluate the changes in uterine luminal fluid proteins during different reproductive stages abattoir derived buffalo genital tracts were classified into proestrus (n=7), estrus (n=7), luteal phase (n=8) and early pregnancy (n=7). The genitalia were dissected and uterine fluid samples were collected in eppendorf tubes. SDS-PAGE analysis was performed on the uterine fluid samples and molecular weights (MWs) of protein bands were estimated by comparing their migration rates on the gel with those of protein markers with known MWs. A total of 24 different protein bands were observed corresponding to MWs varying from 10 to 210 kilodaltons (kDa) during different phases of reproduction with some proteins being specific to some stage of reproduction. Proteins with MWs between 90 and 160 kDa were not found in any of the samples. During proestrus, proteins with MWs between 75-80 kDa were absent whereas they were present during all other stages studied (estrus, luteal stage and early pregnancy). Proteins with high MWs (205-210 kDa) were observed during estrus only. Proteins with MWs between 10-35 kDa were specific to luteal phase and early pregnancy and proteins with MWs 15-20 kDa and 40-45 kDa were specific to pregnancy. It was concluded that the proteins in the uterine luminal fluid change during different reproductive stages in buffalo with low MWs proteins being common during luteal phase and pregnancy and high MWs proteins common during follicular phase.

Keywords | Buffalo, Uterine luminal proteins, Estrus, Pregnancy, Luteal phase.