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Journal of Animal Health and Production

Research Article
J. Anim. Health. Prod. 5(4): 165-171
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Sandeep Kumar Sharma1, 2*, Vikas Galav1,3, Manish Agrawal1,3, Farah Naz Faridi1, Brajesh Kumar3

1Office of the RKVY project Epidemiological Mapping of Antimicrobial Resistance (EMAMR); 2Department of Veterinary Microbiology and Biotechnology; 3Department of Veterinary Pathology, Post Graduate Institute of Veterinary Education and Research (PGIVER), Jaipur, Rajasthan University of Veterinary and Animal Sciences (RAJUVAS), India.

Abstract | For determining the multi- drug resistance pattern of bacterial flora of poultry origin, six caecal, two air sacs and one tracheal sample from necropsy of poultry birds (broiler chicken) including one feed sample were collected from poultry farms of Jaipur division. Four different bacterial species name as viz. Escherichia coli (38.88%), Pseudomonas aeruginosa (27.77%), Staphylococcus aureus (22.22%) and Streptococcus spp. (11.11%) were obtained from processed samples. Escherichia coli were hundred percent resistant to ampicillin, gentamicin, tetracycline, doxycycline hydrochloride. The only antibiotic to which E. coli isolates were susceptible (57.14%) was ampicillin sulbactam. Pseudomonas aeruginosa showed 100% resistance to ceftriaxone, meropenem, ciprofloxacin, erythromycin and colistin while 60% were sensitive to ampicillin sulbactam, ceftazidime, cefoperazone and rifampicin. The isolates of Staphylococcus aureus and Streptococcus spp. were 100% resistant to most of screened antibiotics and retained sensitivity to only imipenem and chloramphenicol. The detected resistance pattern indicated the gross severity of problem and underline that for preventing the spread of bacterial resistance, it is critically important to have regulated antibiotic usage policies and surveillance system for implementing effective control of multidrug resistant organisms. Further the study suggested molecular screening of these isolates in regard to antibiotic resistance genes.

Keywords | Bacterial flora, Multidrug, Resistance, Poultry, Necropsy