Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 4 (2): 42 -49
http://dx.doi.org/10.14737/journal.jahp/2016/4.2.42.49
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Rana Hussein Raheema

Microbiology Department, College of Medicine, Wasit University, Iraq.

*Correspondence | Rana Hussein Raheema, College of Medicine, Wasit University, Iraq; Email: firas_rashad@yahoo.com


Abstract
This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatinine, serum uric acid and serum total protein in normal and Staphylococcus aureus infected mice. Thirty seven milk samples were collected from Al-Kut city from cows suffering acute mastitis. After biochemical tests and API Staph System it was revealed that 19 out of 37 milk samples contained S. aureus. Ethanolic extract of pomegranate peels was prepared and the highest in vitro antibacterial effect was observed by 300mg/mL against S. aureus with 30.00 ± 0.57 mm of the diameter zone. Thirty lactating mice were divided into three groups. Group (1): negative control (Normal). Group (2): positive control, mice were infected by intramammary injection of S. aureus at 7, 15, 21 and 30 days and left untreated. Group (3): mice infected with intramammary injection of S. aureus and treated orally with 300 mg/kg BW of ethanolic extract of pomegranate peel after 48 h of infection for 15, 21 and 30 days. At the end of experiment, blood samples were taken from anaesthetized mice then the mice were killed for histopathological examination and measured the following: serum glucose, creatinine, serum uric acid and serum total proteins. Results showed significant increase (P <0.05) in the levels of serum glucose, creatinine, serum uric acid and serum total proteins in positive control mice as compared with negative control while pomegranate peel treated group showed a significant (P <0.05) decrease in the levels of serum glucose, creatinine, serum uric acid and serum total proteins. Histological section in mammary gland showed clear pathological effects of S. aureus as compared with those treated with pomegranate peel extracts.

Keywords | pomegranate peel extract, Mice, Mastitis, Biochemical parameters

Editor | Asghar Ali Kamboh, Sindh Agriculture University, Tandojam, Pakistan.

Received | December 19, 2015; Revised | January 05, 2016; Accepted | January 07, 2016; Published | February 05, 2016

Citation | Raheema RH (2016). Effect of pomegranate peel extract on some biochemical and histopathological parameters in experimental induced mice with Staphylococcus aureus. J. Anim. Health Prod. 4(2): 42-49.

ISSN | 2308–2801

Copyright © 2016 Raheema. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.