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Journal of Animal Health and Production

Research Article
J. Anim. Health Prod.1 (2): 20 - 23
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Sant Prasad Tyagi, Rajesh Kumar Joshi * and Namita Joshi
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, N.D. University of Agriculture and Technology, Kumarganj- 224229, Faizabad (U.P.), India
*Corresponding Author: rkjoshivet26@gmail.com

ABSTRACT
Pathogenic Staphylococcus aureus was isolated from the cases of subclinical mastitis in lactating cows. A total of 214 quarter milk samples were screened of which, 110 (51.40%) samples were found positive for subclinical mastitis (SCM) using California Mastitis test (CMT). Isolation of Staph. aureus was attempted in 68 samples, comprising 52 distinct and 16 strong CMT positive samples. All 68 samples yielded Staph. aureus. All the isolates were catalase positive while 54 (79.41%) and 56 (82.35%) isolates showed positive slide and tube coagulase test, respectively. On sugar fermentation all the isolates were positive for mannitol fermentation, while 59 (86.76%) and 53 (77.94%) isolates fermented glucose and lactose. On sheep blood agar, 48 (17.58%) isolates showed β-haemolysis and 59 (86.76%) samples were positive for nitrate reduction test. Coa gene was detected in all the Staph. aureus while 22 (32.35%) isolates revealed Spa (X region) gene. All the isolates were sensitive to Cefotaxime while highest number of isolates 63 (92.64%), were found resistant to ampicillin, followed by 20 (29.41%) for tetracycline, 17 (25.00%) for ofloxacin, 11 (16.17%) for lincomycin and 09 (13.23%) for ciprofloxacin, respectively.

Key Words: Staphylococcus aureus , Sub Clinical Mastitis, California Mastitis test, Antibiotic resistance, Polymerase Chain Reaction