Journal of Animal Health and Production

Short Communication
J. Anim. Health Prod. 2 (1): 5 - 7
http://dx.doi.org/10.14737/journal.jahp/2014/2.1.5.7
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Deepak Sharma, Subodh Kumar*, Sitangsu Mohan Deb
Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India
*Corresponding author: subkum@gmail.com

ABSTRACT
An attempt was made to ascertain polymorphism in three fragments of 117, 167 and 142 bp size, corresponding to exon 8, 10 and 13, respectively of CD18 gene in 45 water buffaloes (Bubalus bubalis) of Murrah breed. Upon digestion with HaeIII, NlaIV and SauI restriction enzymes, all DNA samples yielded similar fragments which indicated the absence of mutant allele. Results indicated that studied buffalo populations lack polymorphism in the CD18 genes.

Key Words: Buffalo, CD18, HaeIII, NlaIV, PCR–RFLP, SauI