Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 9(s1): 121-127
Http://dx.doi.org/10.17582/journal.jahp/2020/9.s1.121.127
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Hala S. Abubakr*, Dalia Iskander, El shafei A.A

Bacteriology Department, Animal Health Research Institute, Dokki, Giza and Zagazig Provincial Laboratory of Animal Health Research Institute

Abstract | Pasteurella multocida is a pathogenic Gram-negative bacterium causing respiratory manifestation in cows and other animals resulting in great economic losses. This work aimed to identify the P. multocida using VITEK2 system and detect the toxigenic strains by one step enzyme-linked immune-sorbent assay (ELISA). For detection of P. multocida, 209 samples including 145 nasopharyngeal swabs (115 from cows with respiratory manifestation and 30 in contact apparent healthy animals) and 64 pneumonic lungs from dead cows were collected and examined. The recovered isolates were identified by both conventional methods and VITEK2 compact system. P. multocida isolates were investigated for toxin production by mouse lethality test and P. multocida toxin-specific monoclonal antibodies using ELISA. Out of 63 presumptive P. multocida colonies, 35 isolates (16.75%) were identified using the conventional methods and animal inoculation test; whereas, 43 isolates (20.57%) were confirmed by VITEK2 compact system and were subsequently tested for toxin production. Out of 43 positive P. multocida isolates, only 14 isolates (32.55%) were proved to be toxigenic by using a mouse lethality test; while with the help of a one-step enzyme immunoassay, 17 toxigenic P. multocida isolates (39.53%) were identified. VITEK2 compact system appeared as a fast and reliable method for identification and detection of P. multocida, and PMT ELISA was observed more sensitive than mouse lethality test.

Keywords | P. multocida, Nasopharyngeal swabs, VITEK2 system, one-step enzyme immunoassay