Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 9(1): 88-93
View Full HTML
Download PDF

Mohamed Abdelfattah Maky1, Eman Sayed Mohammed2*

1Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83522, Egypt; 2Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, Qena 83522, Egypt.

Abstract | Sarcocystis is a zoonotic protozoan parasite that can be transmitted through the ingestion of undercooked/raw meat contaminated with mature cysts containing bradyzoites. The present study was conducted to investigate the prevalence of sarcocystosis in imported and processed meat products for first time in Qena province, Egypt. A total of 140 specimens of Brazilian imported frozen beef, beef burger, sausage, luncheon, minced meat, kofta (meat balls) and pastrami (20 for each) were collected from several markets in Qena. Specimens were examined macroscopically, by naked eyes, and microscopically, via peptic digestion technique, for detecting Sarcocystis. The identification of Sarcocystis spp. was done by the use of PCR targeting the 18S rRNA gene as well as sequencing of the obtained nucleotides. Our result showed that 58.57% of specimens had Sarcocystis by pepsin digestion technique, while no macroscopic cysts could be detected. The infection rate was 90.0% in imported meat, 75.0% in minced meat, 65.0% in beef burger, 60.0% in luncheon, 55.0% in kofta, 45.0% in sausage, and 20.0% in pastrami. The recovered Sarcocystis species were identified as S. cruzi, S. hominis, S. hirsuta and S. fusiformis. The current work revealed that the high prevalence of microsarcocystosis in imported and processed meat products reflects the potential role of dogs, definitive hosts, rather than cats in the transmission of Sarcocystis spp. in Qena. Interestingly, S. hominis has a zoonotic importance and could be identified in pastrami. Moreover, it is highly recommended that hygienic measures should be applied during meat processing and the consumption of undercooked meat products should be avoided.

Keywords | Egypt, prevalence, meat samples, PCR, Sarcocystis