Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 9(s1): 56-60
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Aqeel Mahdi Muhammad Al-Ebrahim1, Waleed Rizk El-Ghareeb2*

1Ministry of Environment, Water and Agriculture in Riyadh, Saudi Arabia; 2Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box: 400, Al-Ahsa, 31982, Saudi Arabia.

Abstract | A total of 120 broiler meat samples (60 breast samples and 60 thigh samples) were examined using a high performance liquid chromatography occupied by mass detector (LC-MS/MS) for determination of fluoroquinolones residues (Sarafloxacin, Danofloxacin, Ciprofloxacin, Enrofloxacin, Ofloxacin, and Marbofloxacin) at Al-Ahsa province. The antibiotics were validated according to the guidelines laid down by the joint FAO/WHO Expert Committee on Food Additives. Incidence of fluoroquinolones residues in examined breast meat samples were 44 (73.33%) for Sarafloxacin, 45 (75%) for Danofloxacin, 49 (81.67%) for Ciprofloxacin, 48 (80%) for Enrofloxacin, 50 (83.33%) for Ofloxacin, 45 (75%) for Marbofloxacin while in thigh muscles were 49 (81.67%), 45 (75%), 49 (81.67%), 48 (80%), 55 (91.67%) and 45 (45%) for Sarafloxacin, Danofloxacin, Ciprofloxacin, Enrofloxacin, Ofloxacin and Marbofloxacin, respectively. Mean±SE of Sarafloxacin, Danofloxacin, Ciprofloxacin, Enrofloxacin, Ofloxacin, and Marbofloxacin were 7.10±0.57, 8.05±0.50 and 24.24±1.82, 23.99±1.80 and 21.88±1.35, 22.98±1.45 and 32.36±4.52, 23.06±2.14 and 13.30±1.72, 14.49±1.87 and 8.21±1.06, 9.93±1.28 μg/kg in breast and thigh, respectively. All examined samples were within permissible limit except for enrofloxacin (one breast sample) and Sarafloxacin (20 and 14 breast and thigh samples, respectively) were higher than permissible limits. The study suggested the judicial use of antibiotics in food producing animals to safeguard human health.

Keywords | Fluoroquinolones, Residues, Broiler, Meat, LC-MS/MS