Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 9(s1): 27-33
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Fathy Dosoky EL- Seddawy*, Mohamed Tayiser Mohamed Samy, Nefissa Helmy Mohamed Mekkawy, Ahmed El-Sayed Behery, Walaa Osama Mohamed Youssef

Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt.

Abstract | Severe spinal cord injury (SCI) is a global disorder in both human and animals. Current study investigated the role of platelets rich plasma (PRP) and bone marrow derived mesenchymal stem cells (BM-MSCs) in the treatment of acute compressive spinal cord injury in rat model. Twenty, non-pregnant female Sprague-Dawley rats about 6 months old with a weight of 250-300 g were subjected to laminectomy at the ninth thoracic vertebra (T9) followed by compression of the spinal cord using mosquito forceps for 15 seconds. Rats were equally divided into four groups; control group, PRP group, BM-MSCs group and PRP + BM-MSCs group. The rats were examined clinically at day 1, 7, 14, 21, and 28 postoperatively with Basso-Beattie-Bresnahan (BBB) scale, and then were sacrified after 28 days post operations for histopathological and immunohistochemical examinations. Gradual improvement of the locomotor function of the hind limbs with higher BBB scores were recorded in PRP and BM-MSCs treated groups in comparison to the control group. The use of PRP+BM-MSCs combination showed better white matter integrity and myelinated axons without glial scar formation than the use of either PRP or BM-MSCs alone and permanent pathological changes of the injured cord was shown in control group. The PRP+BM-MSCs combination showed poor immunogenic reaction for glial fibrillary acidic protein (GFAP) than all other groups and the control group showed higher immunoreactivity for GFAP with glial scar formation. It could be concluded that the locomotor activity of the hind limbs were improved after PRP and BM-MSCs treatment with limiting the pathological changes occurring after SCI.

Keywords | Spinal cord, Laminectomy, Platelets rich plasma, Rat, PRP, BM-BMSCs, GFAP