Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 8(4): 183-188
Http://dx.doi.org/10.17582/journal.jahp/2020/8.4.183.188
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Emad Abdel-Hamied, Mourad Mahmoud Mahmoud*

Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni‑Suef 62511, Egypt.

Abstract | This study was designed to evaluate the alterations in oxidative stress status, antioxidants profile, leucogram and some biochemical parameters in Holstein-Friesian (H-F) dairy cows suffered from acute clinical mastitis. Thirty-nine lactating H-F cows were included in this experiment. According to the findings of the clinical examination, the animals were allocated into 2 groups. The first group consisted of 26 cows affected with acute clinical mastitis, while the 2nd group consisted of 13 apparently healthy cows with negative reactions to California mastitis test (CMT) were served as control group. Physical examination of the selected animals including milk and udder was done. Blood, serum and plasma samples were obtained. Antioxidants, Oxidative stress, leucogram and some biochemical indicators were estimated. Malondialdehyde (MDA) level was significantly increased (P<0.05) in mastitic cows than controls along with nonsignificant increase (P>0.05) in the hydrogen peroxide levels in the same group. Catalase (CAT) activities and total antioxidant capacity (TAC) levels were significantly decreased (P<0.05) in the mastitic group meanwhile, glutathione-s-transferase (GST) activities were insignificantly reduced in the same group. Significant elevated values (P<0.05) of total leucocytic count (TLC) along with significant reductions (P<0.05) in lymphocytes were observed in mastitic cows. Significantly reduced levels (P<0.05) of total protein (TP), globulin, calcium and phosphorus were recorded in the mastitic group as compared to controls. In summary, acute clinical mastitis in H-F cows is obviously associated with marked systemic involvement, typically severely swollen painful udder, and abnormal milk. It is also complemented with significant oxidant/antioxidant, biochemical and leukogram variations that could be used as useful indicators for dairy cows pathological status (mastitis). Oxidant-antioxidant changes explained that cows affected with acute mastitis are accompanied by marked oxidative stress that could be used as a biomarker to check the udder health status.

Keywords | Mastitis, Oxidative stress, TAC, MDA, Catalase