Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 8(1): 19-26
Http://dx.doi.org/10.17582/journal.jahp/2020/8.1.19.26
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Veli Gülyaz1*, Fahriye Saraç2, Can Çokçalışkan3, Esra Satır2, Beyhan Sareyyüpoğlu3, Abdullah Arslan3, Serdar Uzar2, Mehmet Karakaya3, Osman Kara3, Gonca Öztap1

1Ministry of Agriculture and Forestry, General Directorate of Food and Control Çankaya, Ankara, Turkey; 2Pendik Veterinary Control Institute, Pendik, Istanbul, Turkey; 3Institute of Foot and Mouth Disease (SAP), Çankaya, Ankara, Turkey.

Abstract | Foot and Mouth Disease (FMD) is highly contagious viral disease of cloven-hoofed animals with huge economic losses because of significant decrease of yield. One of the most basic methods in the fight against FMD is vaccination in many countries. Trivalent or tetravalent inactivated FMD vaccines are used in the fight against FMD extensively. Although, FMD vaccine potency mainly depends on FMD antigen amounts in the vaccine composition as micrograms, it has been required to increase immunity against inactivated FMD vaccines with some immunomodulators or immunostimulants like some bacterial or viral antigens. Therefore, this project was designed to investigate the immune-stimulating effect of ecthyma virus in sheep when it was applied together with FMD vaccine either in same formulation or simultaneous injections at different sites of the body. Level of immunity was measured by virus neutralization test (VNT) and liquid phase blocking ELISA (LPBE). According to our results, the highest antibody titers of virus neutralization (VN) and LPB-ELISA were obtained by single administration of FMD vaccine, followed by simultaneous and combined administrations respectively at both day 30 and 60. However, this difference was statistically non significant (p < 0.05). Thus, it could be concluded that the use of ecthyma virus as an immune stimulant did not cause an increase in antibody level against FMD virus.

Keywords | Foot and mouth disease, ecthyma, Immunity, Vaccine