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Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 7(3): 106-112
Http://dx.doi.org/10.17582/journal.jahp/2019/7.3.106.112
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Jacques Danho Monney1*, Valery Edgard Adjogoua2, Yahaya Karamoko1, Marius N’takpé Adagba2, Agbaya Akran2

1Nature Sciences Training and Research Unit, Animal Biology and Cytology Laboratory, Nangui Abrogoua University. 02 BP 801 Abidjan 02, Ivory Coast; 2Virology departement of Institut Pasteur, 01 BP490 Abidjan 01, Ivory Coast.

Abstract | Bovine Rotavirus (BRV) is the major cause of gastroenteritis and diarrhea in newborn calves. Every year, Rotaviruses cause enormous economic losses. In current investigation, a total of 145 fecal samples were collected from both diarrheic (n=135) and non-diarrheic (n=10) neonatal calves. Samples were collected from diarrheal calves up to the age of three months, from bovine farms located in Abidjan district during 2015 to 2017. All the samples were screened by using conventional One step reverse transcription polymerase chain reaction (RT-PCR) for VP7 and VP4 genes. The results exhibited that 34.8% (47/135) samples were positive for both VP7 and VP4 genes. All non-diarrhoeic samples were negative for BRV. G10 was the most prevalent genotype, accounting for 61.7% (29/47). The detected P types were P[11] and P[5], accounting for 36.1% (17/47) and 23.4% (11/47) respectively. The most common genotype circulating among bovine population of Abidjan district is G10P[11] for 34% (16/47) and followed by G10P[5] for 8.5% (4/47). The results of this study suggest that these two types can be used as dominant strains for the formulation of an appropriate vaccine against bovine Rotavirus in the Abidjan district, Ivory Coast.

Keywords | Bovine, Rotavirus, RT-PCR, Genotype, Abidjan.