Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 10(1): 139-144
 
Figure 1

Photomicrograph of the rabbit uterus at various development stages. (A) 0day, (B) 2weeks, (C) 4weeks, (D, E) 16weeks, (F) 18 days’ pregnant rabbit uterus. Me, mesenchymal layer; L, Lumen; L.E, Lamina epithelialis; M, Muscular layer; S, Serosa; L.P, Lamina Propria; Arrow: Shallow epithelial invaginations; Arrow head, Tubular structure; E.F, Endometrial folds; I.C, Inner circular layer; O.L, Outer longitudinal layer; B.V, Blood vessels; G.E, Glandular epithelialis; E.S, Endometrium stroma; Scale Bar: 50 μm (A, B, C, E); 100 μm (D, F) (Hematoxylin and Eosin stain). 

Figure 2

Assessment of mRNA Levels by RT-PCR during the development of rabbit uterus. (A) Relative expression of mRNA levels for FSHR (B) Relative expression of mRNA levels for LHR. The different letters above the bars reflect the significant differences between the different uterine developmental stages (P< 0.05). Data presented in form of mean ± SEM. Abbreviations: FSHR, Follicular stimulating hormone receptor; LHR, Luteinizing hormone receptor; 0D, 0 day; 2W, 2 Weeks; 4W, 4 Weeks; 16W, 16 Weeks; 18dpc, 18day post-coitum. 

Figure 3

Uterine FSHR and LHR Protein Levels by using western blot (A) Immunodetection of FSHR and LHR and β-actin (control) at different ages. (B) FSHR/β-actin densitometrical assessment (C) LHR/ β-actin densitometrical assessment. The different letters above the bars reflect the significant differences between the different uterine developmental stages (P< 0.05). Data presented in form of mean±SEM. Abbreviations: FSHR, Follicular stimulating hormone receptor; LHR, Luteinizing hormone receptor; 0D, 0 day; 2W, 2 Weeks; 4W, 4 Weeks; 16W, 16 Weeks; 18dpc, 18 day post-coitum.