PCR detection of B. caballi and T. equi with a set of primer combinations (Bec-UF1 and Bec-UR): M= 100 bp DNA marker; Lanes 1-2=B. caballi in approximately band size 876bp; lanes 3-6= T. equi in approximately band size 930bp; Lane N) negative control. 

Gel electrophoresis image showing: lane M) 100bp DNA ladder; Lane P) positive control for T. equi and B. caballi; Lane1-4) multiplex PCR detected T. equi and B. caballi in approximately band size 392 and 540 bp respectively; Lane N) negative control. 

Rooted phylogenic tree of Theileria equi as interfered from partial sequences of 18S rRNA gene. Black diamond indicated sequences obtained from the present study; others represent sequences from references. 

Rooted phylogenic tree of Babesia caballi as interfered from partial sequences of 18S rRNA gene. Black diamond indicated sequences obtained from the present study; others represent sequences from references.