Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(9): 933-939
 
Figure 1

Protein analysis of O. felineus antigens by SDS-PAGE and visualization by Silver Quest Staining Kit: (A) ES-Ag; (B) S-Ag; (C) E-Ag. Line 1, molecular markers; line 2, electropherogram of antigens. 

Figure 2

Antigenicity of O. felineus antigens in indirect-ELISA.

Figure 3

Dot immunoblot patterns of O. felineus antigens in sera of infected and control dogs: (A) ES-Ag; (B) S-Ag; (C) E-Ag. Line 1, 2, 3, 4, 5, sera of infected dogs; line 6, sera of the control dog. The numbers on the left indicate to antigen dilutions that reacted with serum.  

Figure 4

Western blot using positive serum with O. felineus antigens. Line 1, ES-Ag; line 2, S-Ag fraction 1; line 3, S-Ag fraction 2; line 4, E-Ag; M, molecular markers. Molecular mass markers are expressed in kDa. The arrows on the left indicate proteins with molecular masses of 105 and 205 kDa that react with positive serum.