Research Article

 

Enhancing Nutritional Quality of Oil Palm Empty Fruit Bunch for Animal Feed by Using Fiber Cracking Technology

 

Anuraga Jayanegara1*, Nabila F. Ardhisty1, Sari P. Dewi1, Antonius2, Roni Ridwan3, Erika B. Laconi1, Nahrowi1, Muhammad Ridla1

1Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Bogor 16680; 2Goat Research Station, Indonesian Agency for Agricultural Research and Development, PO BOX 1, Galang, Sei Putih 20585;3Biotechnology Research Center, Indonesian Academy of Sciences, Jl. Raya Bogor Km 46, Cibinong, Bogor 16911, Indonesia.

 

Editor | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India.

Received | October 06, 2018; Accepted | November 20, 2018; Published | December 29, 2018

*Correspondence | Anuraga Jayanegara, Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Bogor 16680, Indonesia; Email: anuraga.jayanegara@gmail.com

Citation | Jayanegara A, Ardhisty NF, Dewi SP, Antonius, Ridwan R, Laconi EB, Nahrowi, Ridla M (2019). Enhancing nutritional qualityof oil palm empty fruit bunch for animal feed by using fiber cracking technology. Adv. Anim. Vet. Sci. 7(3): 157-163.

DOI | http://dx.doi.org/10.17582/journal.aavs/2019/7.3.157.163

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2019 Jayanegara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

Introduction

 

Indonesia is currently the world largest producer of oil palm products especially in the form of crude palm oil. This is possible since total oil palm plantation area in Indonesia is the largest among other countries, i.e. 8,150,000 ha, followed by Malaysia (4,620,000 ha), Thailand (720,000 ha), Nigeria (440,000 ha), Colombia (354,000 ha) and others (Garcia-Nunez et al., 2016). Oil palm plantation, apart from its main products, also produces significant amount of residual biomass such as oil palm trunk, oil palm frond, oil palm empty fruit bunch (OPEFB), kernel shell, mesocarp fiber and palm oil mill effluent (POME). Production of palm oil is approximately 10% from total biomass and the remaining is regarded as residual biomass (Ooi et al., 2017). Further, considering conversion factors from Stichnothe and Schuchardt (2010), processing of 100 kg of fresh fruit bunch (FFB) would result 20 kg of crude palm oil and 23 kg of OPEFB. On dry matter basis, two thirds of oil palm residue is originated from oil palm trunk and oil palm frond whereas one third is derived from FFB processing residues (Sulaiman et al., 2010). Such huge amounts of oil palm residues indicate their potency to be used as animal feeds particularly for ruminants since these residues (except POME) generally contain high proportion of fiber (cellulose, hemicellulose and lignin) but low proportion of protein (Ooi et al., 2017).

 

With regard to OPEFB, the biomass was reported to contain 15±8.9% lignin, 43±15.1% cellulose and 21±6.3% hemicellulose, and 0.6±0.4% nitrogen (or equal to 3.75±2.5% crude protein) (Garcia-Nunez et al., 2016). Such nutrient profile is of lower quality in comparison to common agricultural residues fed to ruminant livestock like rice straw (Muhammad et al., 2014; McCan et al., 2017) or oil palm frond (Imsya et al., 2013; Ebrahimi et al., 2015). A feeding experiment on sheep demonstrated that replacement of field grass by fermented OPEFB decreased dry matter intake, average daily gain and nitrogen retention of the animals by 16.4, 70.0 and 42.5%, respectively (Akbar, 2007). It is therefore apparent that OPEBF should be treated through delignification process by means of biological, physical and/or chemical treatment prior to further use (Tsabitah et al., 2014). Urea treatment is able to concomitantly break down lignocellulose component (Van Soest, 2006; Laconi and Jayanegara, 2015) and add certain amount of nitrogen for microbial protein synthesis in the rumen (Gunun et al., 2016). However, conventional urea treatment is relatively time consuming and requires about 3-7 weeks before the feeds can be consumed by the animals (Jayanegara et al., 2017). In our previous study, combination between urea treatment at 1% addition level and elevated temperature and pressure (121oC, 1.4 atm) for 30 min using an autoclave increased dry matter- and organic matter digestibility of rice straw by 23.3 and 25.6%, respectively (Jayanegara et al., 2017). Such treatment was better in comparison to conventional urea treatment in which the rice straw was incubated for four weeks.

 

Such treatment combination may also be applied to OPEFB biomass in order to enhance its nutritional quality. However, it has to be noted from that experiment that the treatment did not decrease the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF) in rice straw (Jayanegara et al., 2017). With the even lower nutritional quality of OPEFB than that of rice straw, it seems that level of urea added, temperature and pressure applied, and incubation period need to be elevated in order to effectively increase OPEFB quality. This experiment therefore aimed to enhance nutritional quality of OPEFB by combining urea treatment and high temperature and pressure (135oC, 2.3 atm), and incubated for 2.5 h.Since such levels of temperature and pressure could not be achieved by a normal autoclave, treatment was performed in a special incubator called Fiber Cracking Technology (FCT).

 

Materials and Methods

 

Sample Preparation and Treatment

The OPEFB sample was collected from PT Perkebunan Nusantara VIII, Cikasungka, Bogor. Sample was chopped, oven-dried at 60oC for 48 h and ground by a hammer mill to pass a 1 mm screen. Ground OPEFB sample was subjected to the following experimental treatments:

 

T1 : Untreated OPEFB (control)

T2 : OPEFB + FCT

T3 : OPEFB + 1% urea + FCT

T4 : OPEFB + 2% urea + FCT

T5 : OPEFB + 3% urea + FCT

T6 : OPEFB + 4% urea + FCT

T7 : OPEFB + 5% urea + FCT

 

The FCT was an incubator with a capacity of 50 l and able to generate high temperature and pressure up to 200oC and 5 atm, respectively. In the present experiment, FCT was set at 135oC and 2.3 atm for 2.5 h. Urea (CO(NH2)2) was solubilized in water at 1:25 kg/l before being added to OPEFB, and the solution was sprayed to the sample, mixed thoroughly and then put into the FCT. All treatments were performed in four replicates.

 

Chemical Composition Analysis

Untreated and treated OPEFB samples were determined for neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin contents according to Van Soest et al. (1991). To determine NDF, 1 g sample was boiled in 100 ml neutral detergent solution for 1 h and the residue was regarded as NDF. Determination of ADF was similar to that of NDF but the solution used was acid detergent solution. Cellulose content was estimated by difference between NDF and ADF. Lignin was determined by adding 72% sulfuric acid to ADF residue and kept for 3 h, and rinsed with hot water and aceton afterwards. The residue was subsequently oven-dried at 105oC for 4 h and burned at 600oC in a furnace. Chemical composition determination was conducted in duplicate.

 

In Vitro Incubation Procedure

Samples of OPEFB were incubated in vitro with rumen fluid and McDougall buffer mixture according to Theodorou et al. (1994). Rumen inoculum (from fluid and solid particle) was obtained from two fistulated Ongole crossbred cattle. Approximately 750 mg sample was inserted into a 125 ml serum bottle and added with rumen fluid and buffer mixture, each of 15 and 60 ml, respectively. Allocation of treatments into serum bottles was according to a randomized complete block design. Incubation was carried out in four replicates (four bottles per replicate,two bottles were incubated for 2 × 24 h and incubation period of the other two bottles was extended to 72 h for measurement of gas production kinetics). Serum bottles werethen immediately sealed with butyl rubber stoppers and aluminum crimp seals. Incubation was conducted at a constant temperature of 39ºC. Total gas production together with methane was recorded during the incubation period. After 24 h, supernatant was collected for determinations of total volatile fatty acid (VFA) and ammonia concentrations (Jayanegara et al., 2016). Residue was further incubated with 75 ml pepsin-HCl 0.2 N solution for another 24 h to measure in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD), and corrected for blanks (Tilley and Terry, 1963).

 

Data Analysis

Data on gas production kinetics during 72 h incubation were fitted with the following (non-linear) exponential and Gompertz models:

 

Exponential model :  

Gompertz model :  

 

where V(t) is volume of gas production (ml) at time t, t is incubation period (h), Vf is asymptotic gas production (ml), e is Euler’s number, L is lag time (h), and k is gas production rate constant (1/h). Goodness of fit of the models was evaluated through coefficient of determination (R2) and mean square error (MSE). Further, analysis of variance (ANOVA) was applied to all data and continued with Duncan’s multiple range test when there was a significant difference among treatments at P<0.05.

 

Results and Discussion

 

Chemical Composition

Treatment using FCT (T2) decreased NDF, ADF, cellulose and lignin contents of OPEFB (Table 1). It is apparent that high temperature and pressure generated by FCT degrade certain fiber component present in OPEFB. Van Soest (2006) described that fiber decrease by high temperature and pressure may occurrsince such condition promotes release of acetyl groups from fiber structure, increases substrate acidity and then finally induces solubilization of fiber component. Combination between FCT and 1-5% urea (T3-T7) further decreased the fiber fractions. Addition 5% urea + FCT (T7) resulted in the lowest NDF, ADF, cellulose and lignin contents of OPEFB among all treatments. In comparison to untreated OPEFB, T7 decreased NDF, ADF, cellulose and lignin by 17.3, 22.9, 25.2 and 15.6%, respectively. During urea treatment, the compound is converted to ammonia and absorbed to OPEFB cell wall to cause severance between lignin and cellulose or hemicellulose complexes (Sarnklong et al., 2010). High temperature and pressure from FCT apparently induced faster reaction for ammonia to degrade lignocellulose complex present in OPEFB. In agreement with our study, pretreatment of OPEFB by using ammonia fiber expansion or AFEX system (1:1 ammonia to biomass loading, 135oC, pressure 35-50 bar, 45 min) decreased its lignin content from 23.38 to 16.84% DM (Abdul et al., 2016). It was further demonstrated that, by using Fourier transform infrared spectroscopy, there were disappearance of peaks at 1738 cm-1 and 1241 cm-1 in the AFEX pretreated OPEFB. These peaks are regarded as carboxylic and carbonyl bonds, respectively, that part of intermolecular ester bonds between carbohydrates and lignin complex. Therefore such disappearance indicated the disruption of lignocellulose complex in OPEFB by AFEX pretreatment (Abdul et al., 2016).

 

Table 1: Neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose and lignin contents (% dry matter) of oil palm empty fruit bunch (OPEFB) treated with Fiber Cracking Technology (FCT).

 

Treatment	NDF	ADF	Cellulose	Lignin
T1	80.5	63.0	47.6	15.4
T2	79.7	58.2	43.1	15.1
T3	78.4	56.7	42.3	14.4
T4	78.2	55.2	40.9	14.3
T5	74.2	54.2	40.1	14.1
T6	69.8	52.7	39.2	13.5
T7	66.6	48.6	35.6	13.0

 

T1, untreated OPEFB (control); T2, OPEFB + FCT; T3, OPEFB + 1% urea + FCT; T4, OPEFB + 2% urea + FCT; T5, OPEFB + 3% urea + FCT; T6, OPEFB + 4% urea + FCT; T7, OPEFB + 5% urea + FCT.

 

In Vitro Gas Production Kinetics

A significant increase of in vitro gas production was observed in the incubation of OPEFB treated with FCT than that of control at various incubation periods (P<0.05; Table 2). Addition of urea at different concentrations combined with FCT further significantly increased gas production (P<0.05) and followed a linear pattern with increasing level of urea. Parameter of in vitro gas production rate showed similar pattern with total gas production parameter (Table 3). Gas production rate of all treatments decreased with increasing length of incubation period. With regard to kinetic parameters of in vitro gas production, higher level of urea addition + FCT led to a higher asymptotic gas production (Vf); this was true when fitted with both exponential and Gompertz models (Table 4). Gas production rate constant (k) was higher with increasing concentration of urea addition when fitted with Gompertz equation, but it was insignificant among treatments in the exponential model. Lag time (L) of gas production was also not significant among treatments. Gompertz model showed better goodness of fit than exponential model as shown by the higher R2 and lower MSE values.

 

Higher gas production of OPEFB treated with FCT or FCT + urea was apparently related to lignocellulose degra-

 

Table 2: In vitro gas production (ml) of oil palm empty fruit bunch (OPEFB) treated with Fiber Cracking Technology (FCT) at various incubation period.

 

Treatment	Incubation period (h)
	2	4	6	8	10	12	24	36	48	72
T1	11a	19a	21a	22a	23a	25a	29a	33a	37a	43a
T2	14b	22b	25b	27b	28b	31b	37b	41b	46b	52b
T3	16c	25c	27c	30c	32c	34c	41c	45c	50c	56c
T4	18d	27d	31d	34d	36d	39d	45d	51d	56d	62d
T5	19d	29d	33e	35d	37d	41d	47e	54e	60e	65e
T6	21e	33e	37f	40e	44e	47e	53f	59f	64f	70f
T7	22f	34e	37f	42f	45e	48e	55f	63g	68g	74g
SEM	0.52	0.72	0.84	0.94	1.04	1.12	1.19	1.38	1.42	1.43
P-value	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

 

Different superscripts in the same solumn are significantly different at P<0.05.

T1, untreated OPEFB (control); T2, OPEFB + FCT; T3, OPEFB + 1% urea + FCT; T4, OPEFB + 2% urea + FCT; T5, OPEFB + 3% urea + FCT; T6, OPEFB + 4% urea + FCT; T7, OPEFB + 5% urea + FCT; SEM, standard error of mean.

 

Table 3: In vitro gas production rate (ml/h) of oil palm empty fruit bunch (OPEFB) treated with Fiber Cracking Technology (FCT) at various incubation period.

 

Treatment	Incubation period (h)
	2	4	6	8	10	12	24	36	48	72
T1	5.6a	4.7a	3.5a	2.7a	2.3a	2.1a	1.2a	0.91a	0.78a	0.60a
T2	7.1b	5.6b	4.1b	3.4b	2.8b	2.6b	1.5b	1.15b	0.95b	0.72b
T3	7.8c	6.1c	4.5c	3.7c	3.2c	2.9c	1.7c	1.26c	1.04c	0.77c
T4	8.8d	6.8d	5.1d	4.2d	3.6d	3.2d	1.9d	1.42d	1.18d	0.86d
T5	9.3d	7.2d	5.5e	4.4d	3.7d	3.4d	2.0e	1.50e	1.24e	0.91e
T6	10.3e	8.3e	6.2f	5.0e	4.4e	3.9e	2.2f	1.64f	1.33f	0.97f
T7	11.2f	8.4e	6.2f	5.3f	4.5e	4.0e	2.3f	1.76g	1.42g	1.03g
SEM	0.26	0.18	0.14	0.12	0.10	0.09	0.05	0.04	0.03	0.02
P-value	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

 

Different superscripts in the same solumn are significantly different at P<0.05.

T1, untreated OPEFB (control); T2, OPEFB + FCT; T3, OPEFB + 1% urea + FCT; T4, OPEFB + 2% urea + FCT; T5, OPEFB + 3% urea + FCT; T6, OPEFB + 4% urea + FCT; T7, OPEFB + 5% urea + FCT; SEM, standard error of mean.

 

Table 4: In vitro gas production kinetic parameters of oil palm empty fruit bunch (OPEFB) treated with Fiber Cracking Technology (FCT).

 

Treatment	Exponential				Gompertz
	Vf	k	R2	MSE	Vf	L	k	R2	MSE
T1	36a	0.125	0.800	20.6	46a	1.14	0.044a	0.943	6.7
T2	45b	0.120	0.851	23.8	51b	1.10	0.055ab	0.958	7.7
T3	49c	0.123	0.873	24.3	54b	1.09	0.063bc	0.957	8.5
T4	54d	0.128	0.877	28.9	60c	1.07	0.065bc	0.953	11.0
T5	57e	0.124	0.864	35.6	64d	1.08	0.062bc	0.958	11.4
T6	62f	0.145	0.890	31.6	66d	1.08	0.092d	0.944	16.0
T7	66g	0.133	0.884	39.2	71e	1.06	0.073c	0.958	14.5
SEM	1.36	0.003			1.20	0.016	0.003
P-value	<0.001	0.128			<0.001	0.852	<0.001

 

Different superscripts in the same solumn are significantly different at P<0.05. T1, untreated OPEFB (control); T2, OPEFB + FCT; T3, OPEFB + 1% urea + FCT; T4, OPEFB + 2% urea + FCT; T5, OPEFB + 3% urea + FCT; T6, OPEFB + 4% urea + FCT; T7, OPEFB + 5% urea + FCT.Vf, asymptotic gas production (ml); L,lag time (h); k, gas production rate constant (1/h); R2, coefficient of determination; MSE, mean square error; SEM, standard error of mean.

 

Table 5: In vitro ruminal fermentation parameters of oil palm empty fruit bunch (OPEFB) treated with Fiber Cracking Technology (FCT) at 24 h incubation period.

 

Treatment	VFA (mmol/l)	NH3 (mmol/l)	IVDMD (%)	IVOMD (%)	CH4/DDM (ml/g)	CH4/DOM (ml/g)
T1	72a	7.6a	34.0a	30.9a	40.1	49.9
T2	77b	8.0ab	39.0b	35.9b	39.1	48.0
T3	85c	8.3bc	42.8c	39.6c	38.9	47.6
T4	93d	8.5c	45.4d	42.3d	38.7	46.9
T5	102e	9.2d	47.3e	44.0e	38.6	46.9
T6	112f	9.2d	49.8f	46.6f	38.8	46.9
T7	120g	9.5d	51.9g	48.8g	37.6	45.2
SEM	2.33	0.10	0.79	0.78	0.50	0.62
P-value	<0.001	<0.001	<0.001	<0.001	0.935	0.638

 

Different superscripts in the same solumn are significantly different at P<0.05.

T1, untreated OPEFB (control); T2, OPEFB + FCT; T3, OPEFB + 1% urea + FCT; T4, OPEFB + 2% urea + FCT; T5, OPEFB + 3% urea + FCT; T6, OPEFB + 4% urea + FCT; T7, OPEFB + 5% urea + FCT.

VFA, volatile fatty acid; NH3, ammonia; IVDMD, in vitro dry matter digestibility; IVOMD, in vitro organic matter digestibility; CH4/DDM, methane per digestible dry matter; CH4/DOM, methane per digestible organic matter; SEM, standard error of mean.

 

radation to result simpler and more fermentable carbohydrate forms. Further, FCT causes expansion of OPEFB cell wall, making easier for rumen microbes to colonize and degrade fiber component. Gas production in an in vitro rumen fermentation system is resulted from microbial degradation on feed components particularly carbohydrate. Different tipe of carbohydrate such as fiber, starch, oligosaccharide and simple sugar (monosaccharide) reveals different rate of gas production. Since starch degradation in the rumen is faster than fiber degradation, its gas production rate is faster as well. Similarly, fermentation of simple sugar is faster in comparison to starch. Fermentation of protein also produces gas but its amount is smaller than that of carbohydrate (Getachew et al., 1998). Lipid contributes to negligible gas production since fatty acids, the main components of lipid, are not degraded and β-oxidized by rumen microbes; they are hydrogenated (in case of unsaturated fatty acids) by certain microbial species to result various fatty acid isomers with lower degree of desaturation (Jayanegara et al., 2012). In addition, gas is also resulted from buffering process of bicarbonate buffer on VFA production during fermentation period (Jayanegara et al., 2017). Decrease of gas production rate with increasing length of incubation period reflects less substrate available for microbial degradation and fermentation.

 

Comparing between exponential and Gompertz in vitro gas production kinetic models, higher goodness of fit of Gompertz model in comparison to exponential model was also observed by Peripolli et al. (2014). The authors compared different gas production models for ruminant diets containing crude glycerol and found that Gompertz model had lower MSE (6.33 vs 24.5) and higher R2 (0.99 vs 0.97) than that of exponential model. Exponential model assumes that gas production rate depends only on available substrate after lag time is accomplished, whereas Gompertz model assumes that specific gas production rate is proportional to microbial biomass (Peripolli et al., 2014). Despite such higher accuracy of Gompertz model, another model namely dual-pool logistic model offers even higher goodness of fit and nutritionally is more relevant for describing profile of in vitro gas production kinetics. In the dual-pool model, gas production is divided into two pools or fractions that represent fast and slow digestion rates and generally the model shows higher accuracy in comparison to first order kinetic functions like exponential and Gompertz model (Groot et al., 1996).

 

In Vitro Rumen Fermentation, Digestibility and Methane Emission

The FCT treatment (T2) significantly increased total VFA concentration, IVDMD and IVOMD of OPEFB in comparison to that of control (P<0.05; Table 5). Increasing level of urea addition led to higher values of VFA, IVDMD and IVOMD. Highest level of urea addition (5%) + FCT(T7) increased VFA, IVDMD and IVOMD of OPEFB by 66.7, 52.6 and 57.9%. The VFA and IVOMD parameters were highly correlated (P<0.001) with total gas production (Figure 1). Ruminal ammonia concentration modestly increased with higher level of urea addition by following a quadratic pattern. Methane emission of OPEFB per unit of digestible DM or OM was unaltered by FCT or urea + FCT treatments.

 

An increase of IVDMD and IVOMD of OPEFB treated with FCT and/or urea is apparently related to lignocellulose degradation and expansion of cell wall as previously explained. Cell wall expansion due to ammonia or urea treatment was also reported by Mahmud-Ali and Bechtold (2015). Therefore there are interconnections (positive relationships) among digestibility, gas production and total VFA concentration. Positive correlation between feed digestibility and in vitro gas production was observed by other authors (Plaizier and Li, 2013). Concomitant increase of total VFA concentration and gas production is expected since both parameters are end products of microbial metabolism in the rumen during nutrient degradation and fermentation (Morvay et al., 2011). Ammonia concentration increase in the incubation of OPEFB treated with urea + FCT may indicate conversion of urea to ammonia under rumen environment. Urea, after being converted to ammonia in the rumen, provides additional advantage by acting as a precursor for microbial protein synthesis and thereafter for supporting protein demand of ruminant livestock (Gunun et al., 2016). Ammonia is an intermediate product of protein metabolism in the rumen; its concentration depends on protein degradation rate, microbial protein synthesis and absorption through rumen villi (Sinclair et al., 2014).

 

IVOMD (%) = 14.0 + 0.622 Gas (ml) (n = 56; P<0.001; R2 = 0.897)

VFA (mmol/l) = 17.7 + 1.753 Gas (ml) (n = 56; P<0.001; R2 = 0.806)

 

Methane emission in the rumen is formed by the action of methanogenic archaea such as Methanobrevibacter ruminantium, Methanosarcina barkeri, Methanosarcina mazei, Methanobacterium formicicum and Methanomicrobium mobile. Main substrates used for methane formation are hydrogen, carbon dioxide and formate (Morgavi et al., 2010). Lack of significant effect of FCT or urea + FCT treatments on OPEFB methane emission is apparently due to high variation of the methane data.On the other hand, an increase of DM or OM digestibility of OPEFB after FCT and/or urea treatment was accompanied by an increase of methane emission as well with relatively similar magnitude, thus contributing to similar methane values among treatments. Reduction of OPEFB fiber by FCT and/or urea treatment may induce a shift of VFA profile towards more propionate that leads to more hydrogen consumption and less methane. However, since total VFA concentration increases as well by the treatments, acetate concentration seems to increase and in turn shift back the methane emission to indifferent value.

 

Conclusion

 

Fiber Cracking Technology seems to be promising for improving nutritional quality of OPEFB especially when being combined with urea treatment. Improvement of OPEFB quality is linear with increasing level of urea and therefore, in the present experiment, use of 5% urea + FCT shows the best nutritional quality among the treatments. Subsequent in vivo evaluation of OPEFB treated with urea + FCT is required to observe whether the treatment is able to effectively improve nutrient utilization and animal performance.

 

ACKNOWLEDGEMENT

 

This research was funded by Indonesian Ministry of Agriculture (Agency for Agricultural Research and Development) through “Kerjasama Kemitraan Penelitian dan Pengembangan Pertanian Nasional (KKP3N)” research grant year 2016.

 

CONFLICT OF INTEREST

 

All authors declare that there is no conflict of interest.

 

Authors Contribution

 

Anuraga Jayanegara designed and supervised the experiment, analyzed the data, wrote and revised the manuscript. Nabila F. Ardhisty and Sari P. Dewi performed the experiment and carried out laboratory analyses. Antonius, Roni Ridwan, Erika B. Laconi, Nahrowi and Muhammad Ridla supervised the experiment and revised the manuscript.

 

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