Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 4(4): 105-110
Http://dx.doi.org/10.14737/journal.jahp/2016/4.4.105.110
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Ambreen Leghari, Asghar Ali Kamboh, Parkash Dewani, Shahid Hussain Abro, Aslam Parvez Umrani, Kanwar Kumar Malhi, Zahid Iqbal Rajput, Shakeel Ahmed Lakho, Iqra Bano, Jan Mohammad Shah

Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan; 2Faculty of Veterinary Sciences, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand 67210, Pakistan; 3Central Veterinary Diagnostic Laboratory, Tandojam, Pakistan.
 

Abstract | Mycobacterium bovis is known to cause significant economic losses to livestock industry in developing countries including Pakistan. Carrier animals with asymptomatic state shed pathogens through many ways as in saliva, nasal or oral secretions, milk, urine etc. In present study, milk and nasal discharge samples were used for the isolation of M. bovis bacterium, in order to explore the most contaminant carrier agent for the spread of bovine tuberculosis and prevalence of disease in the study area. A total of 160 cattle were selected randomly from Hyderabad and Tando Allahyar districts of Sindh province to collect nasal discharge (n= 160) and milk (n= 120) samples that were used for the isolation of M. bovis using Lowenstein-Jensen medium. Risk factors like age, breed, sex, type of farming, pregnancy status, parity, milk production and stage of lactation, associated with bovine tuberculosis were considered as epidemiological risk factors and the data regarding was collected on predesigned proforma. Results showed that, incidence of M. bovis was found higher (p ˂ 0.05) in nasal discharge (1.875%) as compared to milk samples (0.833%). An overall prevalence of 1.42% was found in both types of samples/districts. Individually a higher (p ˂ 0.05) number of M. bovis isolates were recovered from Hyderabad (2.143%) than Tando Allahyar district (0.714%). In Hyderabad district, no any isolate found from female whereas unlike Hyderabad district, no any sample taken from male cattle of Tando Allahyar district found positive. The role of risk factors analyzed during the study were found statistically non-significant (p > 0.05) for both districts. In brief, this study indicated that apparently healthy cattle shed more M. bovis pathogens in nasal secretions as compared to milk. Moreover, both Hyderabad and Tando Allahyar districts, prevailing bovine tuberculosis which is relatively higher in district Hyderabad than district Tando Allahyar.

Keywords | Mycobacterium bovis, Cattle, Culture, Bovine Tuberculosis, Hyderabad, Tando Allahyar