Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 9(s1): 42-48
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Fatma Omara2, Sayed A. Aziz1, Sawsan M. El-Sheikh1, Mahmoud A. A. Said2*

1Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Egypt; 2Zagazig University Hospital, Zagazig University, Egypt.

Abstract | Adverse drug reactions are still facing difficulties until these days. Azithromycin is commonly prescribed for serious infectious diseases of farm animals caused by gram positive cocci and gram negative bacilli. Some data reported that azithromycin has a potential interaction with etoricoxib. The objective of this study was to investigate the possible hepatoprotective effect of ascorbic acid against hepatotoxicity due to azithromycin- etoricoxib interaction using biochemical and histopathological approaches. One hundred and eight adult male albino rats were divided into six equal groups Viz., control, ascorbic acid, azithromycin, etoricoxib, azithromycin+etoricoxib and azithromycin+etoricoxib+ascorbic acid. The results showed a significant (p < 0.05) decline in aspartate transaminase (AST) and alanine transaminase (ALT) level of rats given ascorbic acid alone and in combination with etoricoxib and azithromycin corresponding to control and etoricoxib/azithromycin groups respectively. The ascorbic acid significantly (p < 0.05) reduced the gamma-glutamyl transferase (GGT) and alkaline phosphatase (ALP) level in rats given combination of etoricoxib and azithromycin. A significant increase (p<0.05) in serum glutathione (GSH), glutathione peroxidase (GPx) and decrease (p<0.05) in malondialdehyde (MDA) was observed in ascorbic acid+etoricoxib+azithromycin group as compared with etoricoxib+azithromycin group. Moreover, ascorbic acid restored the liver histomorphological architecture deteriorated by azithromycin+etoricoxib to its normal state. It was concluded that ascorbic acid could prevent hepatocellular damage caused by azithromycin and etoricoxib.

Keywords | Liver, Ascorbic acid, Azithromycin, Etoricoxib, Histopathology