Journal of Animal Health and Production

Research Article
J. Anim. Health Prod. 9(s1): 84-89
Http://dx.doi.org/10.17582/journal.jahp/2020/9.s1.84.89
View Full HTML
Download PDF

Yara F. El-Basrey1*, Rehab. I Hamed2, Mahmoud Mohamed1,3, Mohamed A Lebdah1

1Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University; 2Department of Poultry diseases, Reference Laboratory for Veterinary Quality Control on Poultry Production, Sharkia; 3Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University.

Abstract | During the last 10 years, many inclusion body hepatitis (IBH) outbreaks across Egypt were reported causing high economic losses to poultry industry. The current study aimed the detection and molecular characterization of IBH in broilers at Sharkia governorate. For identification and genotyping of fowl adenoviruses related to IBH in broiler flocks, liver samples representing 40 broiler flocks were collected and tested for FAdVs based on hexon gene detection using polymerase chain reaction (PCR). We recorded most loses among broilers at 3-5 weeks of age. Only depression, ruffled feather were the observed signs with varied mortality (8-14%). On necropsy, liver was yellowish, friable, enlarged and hemorrhagic. For identification and genotyping of Fowl adenoviruses related to inclusion body hepatitis (IBH) in broiler flocks at Sharkia governorate, liver samples representing 40 broiler flocks were collected and tested for FAdVs based on hexon gene detection using polymerase chain reaction (PCR Out of the forty examined broiler flocks, three flocks (7.5%) were recorded PCR positive for Aviadenoviruses. Based on hexon loop-1 gene analysis, the nucleotide sequence identity of the three positive samples (EG101/2018, EG102/2018 and EG103/2018) was >97.7% and genetically was closer to reference strains serotype 2 (FAdV-2), the three examined strains shared a high degree of nucleotide identity (97.6-99.6%). Using neighbor joining for the phylogenetic tree construction depending on hexon gene nucleotides and amino acids sequences, our strains were clustered in the same clade with FAdV type D. This study help to extend the current knowledge about the currently circulating IBH viruses and to develop vaccination and control strategy.

Keywords | Aviadenovirus, Chicken, Inclusion body hepatitis, PCR, Sequencing.