Figure 3
PCR amplification of DNA extracted from blood specimens of Theileria-infected cattle using the specific primer pair, Cytob1, targeting the mitochondrial cytochrome b gene. Note the diagnostic band appeared as 312 bp. Lane M revealed 100-bp ladder= DNA marker. Lanes 1-10 indicated 10 positive animals (animals are numbered as 23, 44, 56, 60, 76, 85, 91, 92, 93 and 98, respectively). Lanes 11 and 12 indicated negative animals (animals numbered 18 and 79)