Seroprevalence of Brucellosis in Sheep and Humans in District

| Brucellosis is a bacterial disease and is caused by genus Brucella. It is highly prevalent zoonotic disease in developing countries including Pakistan. This study was conducted to determine the prevalence of brucellosis in sheep and humans of district Kohat, Khyber Pakhtunkhwa. For this purpose, one hundred blood samples from each humans and sheep (50 each from both sexes) were randomly collected at three different tehsils (Lachi, Seni Gumbat and Kohat) of district Kohat. The serum samples were tested for presence of anti-Brucella antibodies by Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT). Moreover, a total of 50 milk samples were also collected from various sheep herds for subsequent conduction of milk ring test (MRT). The estimated prevalence of ovine brucellosis was 12.12%, 09% and 08.82% in Lachi, Seni Gumbat and Kohat, respectively, with an overall prevalence of 10% in the district. Whereas, SAT and RBPT demonstrated the prevalence as 7% and 6%, respectively, in humans. The results of current study validated that brucellosis was widespread in district Kohat with a relatively higher prevalence being recorded in ewes and occupationally exposed women than their male counterparts. Furthermore, the prevalence of brucellosis was significantly higher in sheep and humans of Lachi and Seni Gumbat tehsils as compared to Kohat. Results of the current study may support the health care organizers to formulate suitable control plans against Brucellosis.


Advances in
health hazard for abattoir workers, farmers, veterinarians and laboratory personnel (Mandel et al., 2005;Rahman et al., 2012 andAsif et al., 2014).Moreover, tremendous economic losses to livestock sector are attributed to brucellosis in terms of abortion, infertility and reduced milk yield (McDermott and Arimi, 2002;Maadi et al., 2011).Brucellosis is widespread in Pakistan owing to lack of appropriate control strategies (Khan et al., 2009;Abubakar et al., 2012).
Ovine brucellosis can be divided into two distinct forms.Like caprine brucellosis, the zoonotic form of ovine brucellosis is caused by Brucella melitensis (Garin-Bastuji, 1994;Samadi et al., 2010;Montasser et al., 2011) and characterized by abortion, stillbirth and placentitis in ewes (Iqbal et al., 2013).This Brucella spp. is considered as highly pathogenic and invasive for humans as compared to Brucella abortus (Acha and Szyfres, 2003).While the non-zoonotic Brucella ovis has been implicated to cause orchitis, epidydmitis and hypospermia in rams (Megid et al., 2010: Lone et al., 2013).Isolation and identification of the causative agent constitutes the most preferred method for presumptive diagnosis of brucellosis.However it is hazardous, time-consuming and difficult to be implemented for large scale diagnosis (Ali et al., 2013).Consequently serological tests based upon the detection of anti-Brucella antibodies are commonly used to diagnose brucellosis (Ferreira et al., 2003).Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) represent the most frequently applied tests for the serodiagnosis of brucellosis (Gul and Khan, 2007).The SAT provides quantitative data on immune responses (Hamidullah et al., 2009).The RBPT is a quick, effective and less expensive test applicable to detect and diagnose brucellosis in large animal herds (Erganis et al., 2005).The sensitivity of RBPT and SAT are 99% and 95.6%, respectively (Barroso et al., 2002;Memish et al., 2002).Milk Ring Test (MRT) is another simple, cheap and widely employed diagnostic test using milk as sample from Brucella suspected animals.Hamidullah et al. (2009)  Although brucellosis has been extensively studied in cattle, buffaloes, dogs and horses, the clear picture of prevalence of ovine brucellosis still exists unstipulated in many developing countries (Yesuf et al., 2010) including Pakistan.Furthermore, the prevalence of occupationally exposed humans is another issue which also required consideration.This project was therefore planned to ascertain the prevalence of brucellosis in sheep and humans of district Kohat, Pakistan, for delineating the significance of Brucella control measures in animal population and awareness about the disease in humans.

study aRea
The study was conducted in District Kohat, comprising of three tehsils i.e., Lachi, Seni Gumbat and Kohat, located in Khyber Pakhtunkhwa province of Pakistan (Figure 1) at geographic coordinates of 33° 35' 13" North latitude and 71° 26' 32" East longitude with an altitude of 508 meters and total area of 2,545 square kilometres.The district is characterized by mountainous topography with a maximum temperature of 40ºC and average annual rainfall of about 638 mm.Kohat Tehsil exhibits relatively high population and developed agricultural infrastructure as compared to other tehsils.

ColleCtion of saMples
One hundred blood samples were randomly collected in 5cc disposable syringes from each humans and sheep (50 each from both sexes) at three different Tehsils (Lachi, Seni Gumbat and Kohat) of district Kohat, Khyber Pakhtunkhwa province of Pakistan.The collected blood samples were kept in slanting position at room temperature to allow clotting.Sera were separated by centrifugation and kept at -20°C until further processing.Later on, the sera were transported in vacutainers (13x100 capacity) to Veterinary Diagnosis and Research Laboratory, Kohat for the analysis of anti-Brucella antibodies.A total of 50 random milk samples from altogether different unvaccinated animals rearing in various sheep herds in the study areas were also collected and stored at 4°C.The milk samples were examined for the presence of antibodies against Brucella spp.using MRT.

Rose bengal plate test (Rbpt)
Rose Bengal plate test (RBPT) was performed according to the procedure described by Zahid et al. 2002.Briefly, the Rose Bengal stained antigen (Veterinary Research Institute (VRI), Lahore) and sera were brought to room temperature.Later on, 40 µl

seRuM agglutination test (sat)
Phenol saline (0.85% NaCl in 0.5% phenol) was used for the preparation of two fold serial dilutions (1:20 to 1:640) of serum samples in different tubes.Equal amount (0.5 ml) of concentrated antigen (VRI, Lahore) was added to each serum-containing tube and the contents were thoroughly mixed.All tubes were incubated at 37 °C for 24 hours.The results were compared with antigen-containing control tube exhibiting 50% agglutination.A titre of 1:40 or above was regarded as positive (Chachra et al., 2009).

MilK Ring test (MRt)
MRT was performed following the method described by Abbas and Aldeewan, (2009).Milk samples and antigen stored at 4°C were brought to room temperature.About 40 µl Hematoxylin stained Brucella melitenesis antigen (VRI, Lahore) was added to each test tube containing 1 ml of milk sample and the con-tents were properly mixed.Test tubes were incubated at 37 °C for 1 hour.Positive reaction was indicated by the appearance of dark blue ring at the upper portion of milk.

antibody titRation
The antibody titre of positive serum samples was also determined using SAT.All serum samples were subjected to two fold serial dilutions (1:20 to 1:160) using four different test tubes (Din et al., 2013).Later on, Brucella melitensis antigen obtained from VRI, Lahore was added to all dilutions and the antigen-antibody interactions observed at various dilutions were recorded.

data analysis
Data thus collected for RBPT, SAT and MRT were probed by applying descriptive statistical technique as described previously by Sikandar et al., (2012).

RESULTS AND DISCUSSION
The overall prevalence of ovine brucellosis was recorded as 10.0% in district Kohat.The prevalence of brucellosis in ewes was recorded to be 12.00%, 10.00% and 10.00% by SAT, RBPT and MRT, respectively in the endemic area.Compared with RBPT and MRT, the prevalence of brucellosis was relatively higher in ewes as determined by SAT (Table 1 and Table 2).While, in rams, SAT and RBPT recorded the seroprevalence as 08.00% and 10.00%, respectively.Regardless of the diagnostic techniques applied, a risk of higher prevalence of brucellosis was evident in ewes as compared to rams.Similar results have also been reported by previous studies (Yesuf et al., 2010;Negash et al., 2012;Rahman et al., 2013).The presence of erythritol in allantoic fluid favours the growth and propagation of Brucella organisms thereby enhancing the susceptibility of female sheep to brucellosis (Yesuf et al., 2010;Rahman et al., 2011).Nevertheless, RBPT detected equivalent (10.00%) seroprevalence in rams as well as in ewes.Our results reinforce the findings of earlier studies demonstrating equal seropositivity in male and female sheep using RBPT (Muma et al., 2006;Iqbal et al., 2013).Both SAT and RBPT demonstrated the prevalence of brucellosis to be 06% in men.The prevalence of brucellosis was recorded as 08.00% and 06.00% in women using SAT and RBPT, respectively.Irrespective of the diagnostic techniques applied, a risk of higher prevalence of brucellosis was obvious in women as compared to men.Previous studies have also reported relatively higher prevalence of brucellosis in women than their male counterparts (Khan et al., 2009;Din et al., 2013 andShahid et al., 2014).The frequent involvement of nomadic and rural females in livestock handling and grazing as compared to their urban counterparts could enhance their susceptibility to brucellosis and other zoonotic problems (Mohmand et al., 2012 andShahid et al., 2014).
Data regarding the prevalence of ovine brucellosis in three different regions (i.e., Lachi, Seni Gumbat and Kohat) of district Kohat (Pakistan) has been illustrated in table 3  proper health facilities, poor hygienic measures, close interaction with animals (for farmers and occupationally exposed persons) and consumption of raw animal products (for farmers) were presumed as the potential risk factors.Nomadic life is characterized by seasonal migration, improper health facilities, close interaction with animals, poor hygienic measures and consumption of raw animal products.Owing to extensive livestock raising, the rural population has been reported to be highly susceptible to brucellosis.Earlier studies also found that poverty and low literacy positively correlated with seropositivity to brucellosis in case of humans (Karimi et al, 2003;Kansiime et al., 2014).The control of human brucellosis is contingent upon the prevention of disease in domestic animals as effective vaccines are not available for human immunization (Thakur et al., 2002;Mukhtar and Kokab, 2008;Rahman et al., 2012).Effective control measures in

Figure 1 :
Figure 1: Map showing the study areas of District Kohat of antigen was added to 40 µl of each serum sample on a clean glass slide and thoroughly mixed by means of a sterilized stirrer.After 4 minutes the slides were checked for presence or absence of agglutination thereby recorded as positive and negative reactions, respectively.
recorded 32.5% ovine/caprine prevalence of Brucellosis during five year study and Mustafa et al. (2011) reported only one case of sheep abortions in Kohat.
Hamidullah et al. (2009)n), respectively.Lone et al. (2013), reported the prevalence of ovine brucellosis as 06.50% in Kashmir.This slightly higher prevalence in the current study on brucellosis could be attributable to geographical variation and altered systems of vaccination and management.However,Hamidullah et al. (2009)reported the prevalence of ovine brucellosis in Kohat as 34.08%.The likely rationale for this lower prevalence in this study could be the involvement of smaller house hold and nomadic sheep herds with less number of animals than larger animal herds at organized farms analysed byHamidullah et al. (2009).Extensive animal farming has been documented as a potential risk factor for ovine brucellosis(Al-Majali,  2005; Yesuf et al., 2010).The prevalence of brucellosis was recorded as 07% and 06% in humans (associated with sheep rearing) by SAT and RBPT, respectively.These values of prevalence are in conformity to the findings ofMohmand et al. (2012) and Rahman et al.  (2012), who reported a prevalence of 06.09% in adult population of two provinces of Pakistan and persons associated with goat farming in Bangladesh, respectively.
However Iqbal et al. 2013  and Negash et al. 2012recorded the seroprevalence of ovine brucellosis as 08.07%and 07% in Ethiopia and NE US Academic Publishers Advances in Animal and Veterinary Sciences September 2014 | Volume 2 | Issue 9 | Page 519

in Animal and Veterinary Sciences September 2014 | Volume 2 | Issue 9 | Page 520Table 1 :
Prevalence of human and ovine brucellosis in Kohat, Khyber Pukhtunkhawa, Pakistan, determined by different diagnostic techniques

Table 2 :
Sex-wise prevalence of human and ovine brucellosis in Kohat, Khyber Pakhtunkhawa, Pakistan

Table 3 :
Prevalence of human and ovine brucellosis in tested regions of Kohat, Khyber Pukhtunkhawa, Pakistan

Table 4 :
Antibody titer of seropositive samples measured using SAT