Early Pregnancy Diagnosis in Dairy Cattle : Economic Importance and Accuracy of Ultrasonography

| Early pregnancy diagnosis is essential for effective management of pregnant animals and early submission of non-pregnant animals for subsequent breeding to reduce calving to conception interval. The present study assessed the accuracy in early pregnancy diagnosis by ultrasonography and estimated the loss due to delayed identification of non-pregnant animals. Transrectal ultrasonography was used to diagnose the pregnancy in dairy animals (n=47) at 30 and 45 days post breeding. The sensitivity and specificity was found to be 92.30% and 97.05%, respectively, on day 30 and reexamination was done at day 45 for confirmation of pregnancy. Animals found positive for pregnancy by ultrasound scanning on day 30 post-breeding had significantly high concentrations of plasma progesterone (> 3ng/ml) on day 20 post-breeding. It was found that pregnancy diagnosis using ultrasonography 30 days after AI can reduce number of open days by 15 days and 30 days compared to per rectal examination, which is generally done on day 45 and day 60 post-breeding, respectively. It was found that one day increase in open period resulted in loss (rearing and milk costs) to the tune of INR 281 and INR 368 in Sahiwal and Karan Fries cows, respectively. It was calculated in Karan Fries cows that if the calving interval is reduced by 1 month due to early pregnancy diagnosis, the expected gain of life time productive days would be around 132 days, which in turn would lead to benefit of about INR 48466. It can be concluded that use of ultrasonography at an early date (at 30 days) post breeding is an effective method for accurate identification of non-pregnant animals to reduce the calving to conception interval in dairy animals. MuzaMil abdullah1, Tushar KuMar MohanTy1, aruMugaM KuMaresan1*, ashoK KuMar MohanTy2, aMbadas raKhaMaji MadKar3, rubina KuMari baiThalu1, MuKesh bhaKaT4 Early Pregnancy Diagnosis in Dairy Cattle: Economic Importance and Accuracy of Ultrasonography Editor | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India. Received | July 29, 2014; Revised | September 10, 2014; Accepted | September 11, 2014; Published | September 27, 2014 *Correspondence | Arumugam Kumaresan, National Dairy Research Institute, Karnal-132001, Haryana India; Email: ogkumaresan@gmail.com Citation | Abdullah M, Mohanty TK, Kumaresan A, Mohanty AK, Madkar AR, Baithalu RK, Bhakat M (2014). Early pregnancy diagnosis in dairy cattle: economic importance and accuracy of ultrasonography. Adv. Anim. Vet. Sci. 2 (8): 464-467 DOI | http://dx.doi.org/10.14737/journal.aavs/2014/2.8.464.467 ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331 Copyright © 2014 Abdullah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 1Livestock Research Centre, Livestock Production Management, National Dairy Research Institute, Karnal – 132001, Haryana India. 2Animal Biotechnology Centre, National Dairy Research Institute, Karnal – 132001, Haryana, India. 3Livestock Production Management, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India. 4Artificial Breeding Research Centre, National Dairy Research Institute, Karnal – 132001, Haryana, India.


Advances in
method used for pregnancy diagnosis, especially under field conditions.By rectal examination, it is difficult to identify the pregnancy before 45 days.Ultrasonography offer a great scope for early pregnancy diagnosis and additionally the cases of early embryonic mortality, which can be misdiagnosed as pregnant by serum progesterone estimation, can be detected by ultrasonography more accurately.Although it is undeIN-Rtood that delay in identifying non-pregnant animals post-breeding leads to economic loss, information on the quantitative estimation of such loss is scanty.Thus, the aim of the present study was to assess the accuracy of ultrasonography in early pregnancy diagnosis and to estimate the economic loss due to delayed identification of non-pregnant animals in an organized dairy herd.

MATERIALS AND METHODS
The study was conducted at Livestock Research Centre, NDRI, Karnal, on 47 animals [32 Karan Fries (KF) and 15 Sahiwal (SW) cows].Animals were reared under loose housing system and fed 10% extra as per NRC (2001) recommendations.Standard management practices, as per the farm schedule, were followed to maintain animals healthy.

TransrecTal-ulTrasonography
The transrectal ultrasonography (Aloka Prosound 2) was done at 30 days post breeding and repeated after 45 days post breeding for confirmation.The examiner maintained standard procedure for ultrasonography.Ultrasound scanning of the uterus and ovaries were made using a 6.5 MHz rectal linear probe (Aloka UST-5820-5, Aloka) for diagnosis and confirmation of pregnancy.

plasMa progesTerone concenTraTion
Blood sample collection was done on day 20 after artificial insemination.The blood sample were immediately centrifuged at 3000 g for 20 minutes to separate the plasma and then the plasma was stored in cryovials at -20 0 C. The samples were analysed for progesterone estimation using Bovine Progesterone Hormone (P 4 ) ELISA test Kit (Endocrine Technologies, Inc. Newark, CA).

sTaTisTical analysis
The sensitivity and specificity of ultrasonography examination were calculated as per Broaddus and De Vries, (2005): Sensitivity = No. of animals correctly diagnosed as pregnant / No. of animals correctly diagnosed as pregnant + Non-pregnant declared wrongly as pregnant Specificity= No. of animals correctly diagnosed as Non-pregnant/ No. of animals correctly diagnosed as Non-pregnant + pregnant declared wrongly as non-pregnant.

calculaTion of losses
Per day losses were calculated by adding per day costs of feed, labour and losses of milk.Wet average of SW (71 milking and 74 dry cows) was calculated as 7.3 litres and for KF (127 milking and 52 dry cows) wet average was 10.5 litres (LRC, NDRI Karnal).The overall losses were calculated by multiplying per day loss with number of open days.Labour rates were kept as per current local rates of Haryana (Labour Law Reporter, 2014).The procurement price for cow's milk was kept at INR 22/litre as per the local prevailing rate.

RESULTS
The transrectal ultrasonography done at 30 days post-breeding showed sensitivity and specificity of 92.30% and 97.05% and re-examination was done on day 45 for confirmation of pregnancy (Table 1).The USG scan of pregnant cows is depicted in Figure 1.Plasma progesterone concentration ranged from 3.19 to 4.97 ng/ml on day 20 after AI in pregnant animals.Non-pregnant animals showed significantly (P<0.05)lower plasma progesterone concentration at 20 days post-AI (0.39 to 0.88 ng/ml) compared to pregnant animals.

expecTed reducTion in nuMber of open days due To early pregnancy diagnosis
By using ultrasonography at 30 days after AI, the non-pregnant animals can be identified at least 15 days and 30 days earlier when compared to the standard per rectal examination that is generally conducted on day 45 and day 60, respectively.

expecTed econoMy of early pregnancy diagnosis
The cost of rearing (feeding and labour cost) for main taining SW and KF cow/day was estimated to be INR

CONCLUSIONS
The use of ultrasonography at an early date is an accurate method for identification of pregnant and non-pregnant animals and may help reduce the calving to conception interval.Transrectal ultrasonography done at 30 days showed sensitivity and specificity of 92.30% and 97.05%, respectively.The economic benefit through early pregnancy diagnosis at 30 120 and INR 137, respectively.The losses due to milk Advances in Animal and Veterinary Sciences August 2014 | Volume 2 | Issue 8 | Page 466 th day post service by USG was INR 4215 in Sahiwal and INR.5520 in Karan Fries cows by reducing the number of open days of around 15 days compared to per rectal examination done on 45 days post service.

Table 1 :
Details of the cows subjected to trans-rectal ultrasonography Figure 1: Ultrasound image of 30 days (A) and 45 days (B) pregnancy in Karan Fries cows.were calculated as INR 161 in SW and INR 231 in KF.It was found that total per day loss due to one day increase in open period due to delay in pregnancy diagnosis in SW and KF cow was INR 281and INR 368, respectively.Early pregnancy diagnosis on 30 th day post service would benefit about INR.4215 for SW and INR.5520 for KF cows by reducing the number of open days of around 15 days compared to per rectal examination done on 45 days post service.

calculaTion of expecTed life TiMe benefiTs froM Kf due To early pregnancy diagnosis
In the present study the economic return due to early pregnancy diagnosis at 30 days by USG was around INR 4215 for SW and INR 5520 for KF cows, which was comparable to the studies reported earlier.